补气通络解毒方含药血清对人BbPG3胰腺癌细胞抑制作用的研究

Experimental research of Buqi Tongluo Jiedu decoction medicated serum inhibiting on growth of BbPG3 pancreatic cancer cell

[目的]观察补气通络解毒方含药血清对人BbPG3胰腺癌细胞抑制作用。[方法]将补气通络解毒方含药血清和空白对照血清分别在体外培养人胰腺癌细胞系BbPG3,MTT法检测各时间段胰腺癌细胞的OD值和细胞增殖率,流式细胞仪检测血清G0/G1、S、G2/M期细胞数。[结果]培养至4h时,细胞存活率为93.5%,2组OD值差异无统计学意义;24h时,细胞存活率为71.2%,2组OD值差异有统计学意义(P<0.05);48h时,细胞存活率为46.9%,2组OD值差异有统计学意义(P<0.05);72h时,细胞增殖率为19.7%,2组OD值差异有统计学意义(P<0.01)。给药组S期百分比明显低于空白对照组,2组差异有统计学意义(P<0.05),空白对照组G0/G1期细胞百分比明显升高,2组比较差异有统计学意义(P<0.05)。G2/M期,2组比较差异无统计学意义,给药组PI值明显低于对照组,差异有统计学意义(P<0.05)。[结论]补气通络解毒方含药血清有明显抑制人胰腺癌细胞增殖的效应。

[Objective]To observe the effect of Buqi Tongluo Jiedu decoction medicated serum inhibiting growth of BbPG3 pancreatic cancer cell.[Methods]Buqi Tongluo Jiedu decoction drug-containing serum and control group serum cultivate human BbPG3 pancreatic cancer cell in vitro respectively.OD value of pancreatic cancer cells and the cell proliferation rate in each period were detected by MTT method,serum the cell number of G0/G1,S,G2/M phase were detected by flow cytometry instrument.[Results]When culture to 4h,the cell survival rate was 93.5%,there was no significant difference between two groups;24h,the cell survival rate was 71.2%,the OD value in both groups have significant difference（P〈0.05）;48h,the cell survival rate was 46.9%,the OD value in both groups have significant difference（P〈0.05）;72h,the cell proliferation rate was 19.7%,the OD value in both groups have significant difference（P〈0.01）.In S phase,the percentage in drug-containing serum group was significantly lower than the blank control group,the two groups have significant difference（P〈0.05）.The control group in G0/G1 phase cell percentage in-creased significantly,two groups have significant difference in statistics（P〈0.05）.G2/M phase,there was no significant difference between two groups,the PI value in drug-containing serum group was obviously lower than the control group,there was significant difference in both group（P〈0.05）.[Conclusion]Buqi Tongluo Jiedu decoction medicated serum has significant effect in inhibiting the proliferation of human pancreatic cancer cell.