生大黄致黑变病结肠的Cajal间质细胞相关研究

Studies on interstitial cells of Cajal of melanosis coli caused by Rhubarb

[目的]观察生大黄致黑变病结肠Cajal间质细胞(ICC)的细胞结构改变及相关神经递质的变化。[方法]建立豚鼠结肠黑变病模型,观察生大黄致豚鼠黑变病结肠ICC的变化及凋亡情况,并检测结肠组织中相关血管活性肠肽(VIP)、P物质(SP)、一氧化氮(NO)的含量。[结果]黑变病豚鼠结肠ICC细胞胞质中线粒体部分空泡状或双层膜结构消失,内质网扩张,部分内质网表面核糖体脱落;结肠组织细胞凋亡数增加;VIP等神经递质数量差异无统计学意义。[结论]长期应用生大黄等蒽醌类药物可致ICC细胞形态的变化,结肠上皮细胞凋亡增加,其变化不排除与黑变病结肠的排便功能下降相关。

[Objective]To observe the changes of interstitial cells of Cajal（ICC）of melanosis coli by Rhubarb.[Methods]To establish the model of guinea pigs colon melanosis,and observe the morphological changes and apoptosis of ICC on melanosis coli induced by Rhubarb in guinea pigs.[Results]The pathological changes of interstitial cells of Cajal appeared as follows：vacuolation or disappearing of double membrane structure of partial mitochondria in cytoplasm,dilatation of endoplasmic reticulum,ribosomes removed from some part of the rough endoplasmic reticulum,and increased the numbers of apoptosis of colon tissue.There was no statistically significant difference in VIP and other neurotransmitters.[Conclusion]Longterm intaking drugs contain anthraquinone can cause the morphological changes of ICC,and increase the apoptosis of colon epithelial cell.This kind of changes cannot exclude the relation of decline in colon melanosis motility function.