芪防鼻敏颗粒对变应性鼻炎大鼠行为学与组织病理学的影响

Effects of Qifang Bimin Particle on the Behavior and Histopathology of Rats with Allergic Rhinitis

目的:探讨芪防鼻敏颗粒对变应性鼻炎(AR)大鼠行为学、鼻腔分泌物嗜酸性粒细胞(EOS)及鼻黏膜组织病理学的影响。方法:SD大鼠随机分为空白对照组、模型组、氯雷他定组(1.7 mg·kg^(-1))、鼻炎康组(0.6 g·kg^(-1))和芪防鼻敏颗粒高(4.6 g·kg^(-1))、中(2.3 g·kg^(-1)),低(1.15 g·kg^(-1))剂量组。采用卵清蛋白(OVA)制备AR大鼠模型,模型建立成功后,灌胃给药,每日1次,连续14 d。对各组大鼠行为学症状进行评分,检测鼻腔分泌物EOS含量,观察鼻黏膜组织病理学变化。结果:与模型组相比,芪防鼻敏颗粒各剂量组行为学评分明显降低(P<0.01),鼻腔分泌物EOS浸润明显下降(P<0.05或P<0.01)。组织病理学结果显示,芪防鼻敏颗粒各剂量组黏膜上皮基本完好,部分可见腺体增生,血管轻度充血,少量EOS浸润,与模型组比较,病理评分显著降低(P<0.05或P<0.01)。结论:芪防鼻敏颗粒可降低AR大鼠行为学评分,减轻鼻腔分泌物EOS浸润,改善大鼠鼻黏膜组织病理学,对AR大鼠起到一定的治疗作用。

Objective： To research the effects of Qifang Bimin particle on the behavior, eosinophils （EOS）in nasal secretion and nasal mueosa pathological histology of rats with allergic rhinitis （AR）. Methods： SD rats were randomly divided into the normal group, model group, Biyankang group （0.6 g·kg^-1）, loratadine group （ 1.7 mg·kg^-1 ）, and high dose group （4.6g·kg^-1）, middle dose group （2.3 g·kg^-1 ） and low dose group（ 1.15 g·kg^-1） of Qifang Bimin particle. AR rat model was induced by ovalbumain （OVA）. After the model was successfully established, the animals were administered continuously for 14 days, once a day. The behavior symptoms were scored, the content of EOS was detected and the pathological changes in the nasal mucosa were observed. Resuits： Compared with those in the model group, the behavior scores and the content of EOS in the nasal secretion of Qifang Bimin particle groups significantly decreased （P 〈 0.05 or P 〈 0.01 ）. Qifang Bimin particle groups had no exposure-related nasal lesion partly with gland hyperplasia, congestion of blood vessel and EOS infiltration, and the pathological scores were notable lower than that of the model group （P 〈 0.05 or P 〈 0.01 ）. Conclusion： Qffang Bimin particle can improve the behavior of rats with AR, and relieve the nasal mucosa pathological changes.