甘草黄酮对乙醇氧化损伤小鼠脑及肝肺组织形态的影响

Effects of licorice flavonoids on the tissue morphology of brain,liver and lung of mice with ethanol oxidative damage

为了观察甘草黄酮对氧化损伤小鼠脑、肝脏、肺脏组织形态的影响,试验把60只小鼠随机分成6组,每组10只,即空白对照组、模型组、维生素C组、甘草黄酮低剂量组、甘草黄酮中剂量组、甘草黄酮高剂量组,除空白对照组外其余各组连续采用甘草黄酮灌胃小鼠42 d,再灌胃乙醇建立乙醇损伤模型,观察不同浓度的甘草黄酮(40,100,150 mg/kg)灌服小鼠后试验现象,并处死小鼠后快速取出全脑、肝脏、肺脏进行H.E.染色,观察组织形态。结果表明:模型组小鼠脑梗死区域可见大量神经元变性、坏死,胞体缩小变形,核固缩,噬神经元现象,脑组织水肿。与模型组相比,甘草黄酮低剂量组小鼠病理改变的严重程度降低,但可见小量神经元变性坏死;甘草黄酮中剂量组小鼠病理改变较模型组轻,神经元变性坏死部分的数量减少,噬神经元现象也明显降低,脑组织水肿明显降低;甘草黄酮高剂量组小鼠梗死区亦可见少量的神经元变性、坏死,见不到"噬神经元现象",脑组织无水肿。甘草黄酮3个剂量组与乙醇氧化损伤模型相比较组织形态明显有差异。说明甘草黄酮对小鼠脑、肝脏、肺脏组织病理学改变具有明显的改善作用,且都呈现明显的剂量依赖效应。

To investigate licorice flavonoids on the tissue morphology of brain, liver, lung of mice with oxidative damage, 60 mice were randomly divided into 6 groups of 10 mice each. The six groups included a blank control group, model group （ MG）, vitamin C group （ VCG）, low dose group （LDG） with licorice flavonoids, middle dose group （MDG） with licorice flavonoids, high dose group （HDG） with licorice flavonoids. In addition to the COG, the other five groups were intragastrically administrated with licorice flavonoids for 42 d, and then intragastrically adminis- tratod with ethanol to establish a mouse model of ethanol damage. The experimental phenomena of mice intragastrically administrated with differ- ent concentrations of licorice flavonoids including 40, 100, 150 mg/kg were observed. The all brain, liver and lungs of mice were taken out quickly after the mice were sacrificed, and then were used for H. E. staining and observation of tissue morphology. The results showed that the degeneration and necrosis of a large number of neurons in cerebral infarction area of mice were seen in the MG, and the cell body reduced to de- formation ; there were pycnosis in the nucleus, and there were neurenophagia and edema of cerebral tissue in the MG. The severity of the patho- logical changes in the mice of LDG with licorice flavonoids was reduced compared with the MG, but the degeneration and necrosis of a small number of neurons was visible. The pathological changes in the mice of MDG with licorice flavanoids were more slightly compared with the MG, and the number of neuron degeneration and necrosis was reduced, the neurenopbagla and edema of cerebral tissue were also significantly re- duced. Small amounts of neuron degeneration and necrosis in infarction area of mice were seen in the HDG with licorice flavonoids compared with the MG, and there were no neuronophagia and edema of cerebral tissue in the HDG. There were significant differences on tissue morpholo- gy between three dose groups with licorice flavonoids compared with the mouse model of ethanol oxidation damage. The results indicate that lico- rice flavonoids have obvious improvement on the pathological changes in the tissues of brain, liver, and lung of mice, and there are significant dose - dependent effects.