基于Actinomyces ruminicola的微态制剂的体外发酵效果

The in vitro fermentation effect of microecological agents based on Actinomyces ruminicola

为了研制一种新的微生态制剂,试验利用分离得到的瘤胃厌氧菌Actinomyces ruminicola(命名为NY2)与痤疮丙酸杆菌混合制成微生态制剂进行体外发酵,研究微生态制剂的体外发酵特性及产挥发性脂肪酸的能力,确定微生态制剂中Actinomyces ruminicola与痤疮丙酸杆菌的最佳混合比例。结果表明:当微生态制剂中Actinomyces ruminicola与痤疮丙酸杆菌混合比为1∶1时,培养瓶中乙酸、丙酸、丁酸和总挥发性脂肪酸的浓度最高值分别达到91.07,41.12,27.58,169.19 mmol/L。该比例微生态制剂可保持瘤胃内容物pH值在6.0左右波动,有利于瘤胃微生物发酵,并能促进乙酸、丙酸等挥发性脂肪酸的生成。

To develop a new kind of microeeological agents, the ruminal anaerobe Actinomyces ruminicola which was isolated and named NY2 was used to mix with Propionibacterium acnes to make microeeological agents for in vitro fermentation. The characteristics of in vitro fermentation of microecological agents and the ability of producing volatile fatty acids were studied to determine the best mixed ratio of the two kinds of bacte- ria in the microecological agents. The results showed that the highest concentration values of acetic acid, propionic acid, butyric acid and total volatile fatty acid in the culture flasks reached 91.07, 41.12, 27.58 and 169.19 mmol/L when the mixed ratio of Actinomyces ruminicola and Propionibacterium acnes in the microacolagical agents was 1 ： 1, respectively. The microecological agents with the mixed ratio could maintain rumen pH to fluctuate around 6.0, which was conducive to rumen microbial fermentation, and could promote the generation of acetic acid, propionic acid and other volatile fatty acids.