咖啡酸锗对小鼠U14瘤的抑制作用及细胞凋亡机制的研究

Study on the inhibition effect of caffeic acid germanium on the U14 tumor in mice and the cell apoptosis mechanisms

为了研究咖啡酸锗对小鼠U14瘤的抑制作用及其细胞凋亡机制,试验采用MG-P染色、透射电镜和免疫组化方法观察了U14瘤细胞的凋亡及瘤细胞中Bax和Bcl-2蛋白表达情况。结果表明:MG-P染色及透射电镜观察可见,咖啡酸锗试验组出现较多的凋亡细胞,可见典型的细胞凋亡形态特征;免疫组化试验显示,咖啡酸锗各剂量组能下调Bcl-2蛋白表达(P<0.05),上调Bax蛋白表达(P<0.01或P<0.05)。说明咖啡酸锗能抑制小鼠U14瘤的生长,并能诱导U14瘤细胞凋亡,这可能是其发挥抗肿瘤作用的主要机制之一。

To study the inhibitory effect of caffeic acid germanium （Ge） on the U14 tumor in mice and cell apoptosis mechanism, the methods of methyl green - pyronin （ MG - P） staining, transmission electron microscope and immunohistochemistry were used to observe the apoptosis of U14 tumor cells and the expressions of Bax and Bcl - 2 proteins in the U14 tumor cells. The results showed that more apoptosis ceils were found in the experimental groups of caffeic acid Ge by MG - P staining and transmission electron microscope, while typical morphological features of apoptotic cells were also found. Immunohistochemistry showed that each dose group of caffeic acid Ge could significantly down - regulate （ P 〈 0.05 ） the expression of Bcl - 2 protein, and extremely significantly or significantly upregulate （ P 〈 0.01 or P 〈 0.05 ） the expression of Bax protein. The results indicate that caffie acid Ge can inhibit the growth of the U14 tumor in mice and induce the apoptosis of U14 tumor cells, which may be one of main mechanisms for the anti - tumor effect of caffeic acid Ge.