早期蕈样肉芽肿微小RNA表达谱的研究

MicroRNA expression profiles in early mycosis fungoides

目的：筛选与早期蕈样肉芽肿（MF）相关的微小RNA（miRNA ）。方法用高通量miRNA PCR芯片检测6例早期MF与6例湿疹和扁平苔藓皮损中miRNA的表达差异。针对差异表达的miRNA，进行13例早期MF、13例湿疹和扁平苔藓皮损组织及Myla细胞株的实时荧光定量PCR（RT-qPCR）验证。结果芯片结果示，相对于对照组，早期MF hsa-miR-378a-5p、hsa-miR-107、hsa-miR-302c-3p显著高表达，差异有统计学意义（P〈0.05）。皮损组织的RT-qPCR验证结果与芯片结果一致。与正常人外周血T淋巴细胞相比，Myla细胞株中hsa-miR-378a-5p、hsa-miR-107显著上调，与芯片结果一致；未见hsa-miR-302c-3p的差异性表达。结论与炎症性皮肤病相比，早期MF存在差异表达的miRNA表达谱。

Objective To screen microRNAs（miRNAs）related to early mycosis fungoides（MF）. Methods A high-throughput miRNA PCR array was used to determine miRNA expression profiles in skin lesions of 6 patients with early MF （early MF group） and 6 patients with lichen planus （control group）, followed by screening of differentially expressed miRNAs between the two groups. Then, real-time fluorescence-based quantitative PCR（RT-qPCR）was performed to verify the differentially expressed miRNAs in lesional specimens from 13 patients with early MF and 13 patients with eczema or lichen planus, as well as in Myla cells and normal human T-lymphocytes. Results The high-throughput miRNA PCR array showed that the expressions of hsa-miR-378a-5p, hsa-miR-107 and hsa-miR-302c-3p were significantly higher in the early MF group than in the control group（all P〈0.05）. For skin lesions, the results from RT-qPCR were similar to those from the miRNA array assay. Compared with normal human peripheral blood T-lymphocytes, Myla cells showed significantly increased expressions of hsa-miR-378a-5p and hsa-miR-107, which was consistent with the results from the miRNA array assay. However, no significant difference was observed in the expression of hsa-miR-302c-3p between the two kinds of cells. Conclusion MiRNA expression profiles in early MF are different from those in inflammatory skin diseases.