寻常性银屑病患者皮损及外周血miRNA表达谱的研究

MicroRNA expression profiles in peripheral blood and skin lesions of patients with psoriasis vulgaris

目的：探讨寻常性银屑病患者皮损及外周血miRNA表达谱系，寻找表达一致的miRNA。方法用基因芯片技术筛选17例银屑病皮损和外周血miRNA，通过验证筛选出差异性miRNA，探讨其与银屑病皮损和面积严重度指数（PASI）的相关性。结果利用Agilent Human miRNA芯片技术,得出银屑病皮损和外周血中相一致的15个miRNA，经过RT-qPCR技术验证，表达一致的miRNA有7个，其中miR-30e-5p、miR-192-5p、miR-17-3p、miR-1227-5p的表达量与银屑病患者PASI评分呈负相关（P＜0.05）；miR-125b-5p、miR-642a-5p、miR-29a-5p与PASI评分无明显相关性（P＞0.05）。结论寻常性银屑病患者皮损组织和血浆中存在着表达一致的miRNA，这些miRNA有望作为评估银屑病患者病情严重程度的生物标记物之一。

Objective To investigate microRNA（miRNA）expression profiles in peripheral blood and skin lesions of patients with psoriasis vulgaris（PV）, and to seek miRNAs consistently expressed in peripheral blood and skin lesions. Methods A miRNA microarray was used to screen differentially expressed miRNAs in skin lesions and peripheral blood samples between 17 patients with PV and 4 healthy human controls, and real-time fluorescence-based quantitative PCR（RT-qPCR）to verify the differentially expressed miRNAs. The correlations of these differentially expressed miRNAs with psoriasis area and severity index（PASI）score were assessed by Pearson correlation analysis. Results The Agilent human miRNA microarray profiling revealed 15 miRNAs consistently expressed in skin lesions and peripheral blood of patients with PV. Of the 15 miRNAs, 7 were verified as consistently expressed miRNAs by RT-qPCR. Among the 7 miRNAs, the expression intensity of miR-30e-5p, miR-192-5p, miR-17-3p and miR-1227-5p was negatively correlated with PASI scores（all P〈0.05）, while that of miR-125b-5p, miR-642a-5p and miR-29a-5p was uncorrelated with PASI scores（all P〉0.05）. Conclusion Some miRNAs are consistently expressed in skin lesions and plasma of PV patients, which are expected to serve as biomarkers for evaluation of psoriasis severity.