叉头框蛋白O1在正畸力介导的牙槽骨改建中的表达改变

The change of FOXO1 expression during alveolar bone remodeling induced by orthodontic force

目的观察大鼠正畸牙移动牙槽骨的改建及叉头框蛋白O1(forkhead box O1,FOXO1)的表达改变,初步探讨FOXO1在正畸力介导的牙槽骨改建中的作用。方法构建大鼠牙移动模型,左侧上颌第1磨牙在50 g力作用下近中移动。分别于牙移动1、3、7 d处死大鼠。通过HE染色及免疫组织化学法观察牙移动不同时间点第1磨牙根分叉区牙槽骨的改建及FOXO1的表达水平。结果正畸力作用后,大鼠上颌第1磨牙不断近中移动。牙移动组根分叉区牙槽骨内破骨细胞较对照组明显增加,牙移动第3 d时破骨活性最强。加载正畸力后,根分叉区牙槽骨内活跃的成骨细胞逐渐增加,成骨活性增强;根分叉区牙槽骨内FOXO1主要表达于成骨细胞内,且随着成骨细胞的增加,FOXO1的表达逐渐增强。结论正畸力介导了根分叉区牙槽骨的改建,FOXO1的表达改变可能与正畸牙移动骨改建相关。

Objective To study the remodeling of alveolar bone and change in expression of forkhead box O1 （FOXO1） during orthodontic tooth movement （OTM） in rat, so as to preliminarily investigate the role of FOXOI in alveolar bone remodeling induced by orthodontic force. Methods The rat OTM models were established and the left maxillary 1st molars were moved with force of 50 g. The rats were executed on the lst, 3rd and 7th day of OTM, respectively. HE staining and immunohistochemical staining were used to observe the remodeling of alveo- lar bone in the inter-radicular region of the 1st molars and expression of FOXO1 at different time points during OTM. Results The I st molars were constantly moved mesially under orthodontic force. There were more osteo- clasts in the alveolar bone of OTM group than that in non-OTM group, and the osteoclasts on the 3nt day of OTM showed the highest activity. The number of active osteoblasts gradually increased in the inter-radicular region of alveolar bone under orthodontic force, with the enhanced osteoblast activity. Expression of FOXOI in OTM groupwas elevated compared with non-OTM group. Most osteoblasts in alveolar bone during OTM were FOXO1 posi- tive, and the expression of FOXO1 was gradually increased with the number of osteoblasts increasing. Conclusion Orthodontic force induces bone remodeling of alveolar bone in the inter-radicular region during OTM, and the change in FOXO1 expression may be related to alveolar bone remodeling during OTM.