荒漠微藻的碳氧转换与调控

The Efficient Carbon-oxygen Transformation and Regulation of Desert Microalgaes

为了提高微藻的生物燃料生产效率及其在密闭环境中的碳氧转换效率,以两株荒漠微藻BG18-3、BE6-2和一株淡水蓝藻7924为研究对象,对其进行逆境条件培养,发现荒漠微藻BG18-3在各种逆境中表现最佳。在静态培养中,荒漠微藻BG1-3也具有明显的优势,其生物量干重达到0.26 g/L,硝态氮和磷酸盐去除率分别为36%和99%。在荒漠微藻BG18-3的通气培养中,生物干重量最高(3%CO_2通气培养16天)达到2.63 g/L,生物量产率为164.0 mg/L·d,出口CO_2浓度最低降到0.04%,O_2净含量增加0.68%,这表明荒漠微藻BG18-3具有较高的碳氧转化效率,具有生产生物燃料的潜质。最后根据18s r DNA分析结果将荒漠微藻BG18-3鉴定为栅列藻Scenedesmus littoralis。

In order to produce biofuels by the use of microalgae and improve the efficiency of carbon-oxygen transfer in a confined environment,the growth of desert microalgae BG18-3,BE6-2 and freshwater cyanobacterial strain 7924 were thoroughly explored in stressed conditions,and microalgae BG18-3 was found to have the best performance in some stressed conditions. Compared to other two strains of microalgae in static culture,desert microalgae BG1-3 had an advantage on microalgae biomass accumulation and nitrogen and phosphorus removal efficiency,of which the biomass dry weight reached 0. 26 g / L,nitrogen and phosphate removal efficiency were36% and 99%,respectively. In the aeration culture of desert microalgae BG18-3 ventilation,the highest biological dry weight（ 3% CO_2 aeration cultured for 16 days） and the biomass yield reached 2. 63 g / L and164. 0 mg / L·d respectively, and the concentration of CO_2 at the outlet dropped to 0. 04%, while the concentration of O_2 increased by 0. 68%,which indicates that the BG18-3 has the potential to be a biological medium in a confined environment. Finally,the BG18-3 was identified as Scenedesmus littoralis according to the results of r DNA 18 s analysis.