双氢青蒿素对下咽癌Fadu细胞凋亡的影响

Effects of dihydroartemisinin on the apoptosis of Fadu cells

目的:探讨双氢青蒿素(DHA)对下咽癌Fadu细胞凋亡的影响。方法:用不同浓度DHA干预下咽癌Fadu细胞,观察DHA对细胞形态的影响;分别于24h或48h后用MTT法检测细胞增殖的变化并计算IC50值。用AnnexinⅤ-FITC凋亡检测试剂盒分析DHA对Fadu细胞凋亡的影响。用Western Blot法测定DHA对Bcl-2、Bcl-xl、Mcl-1、Bax和C-PARP凋亡相关基因的影响。结果:一定浓度的DHA可显著改变细胞的形态学变化,抑制下咽癌Fadu细胞增殖且明显增加凋亡细胞的数量,该变化与干预浓度和时间呈依赖关系。进一步研究结果提示,DHA可显著抑制Bcl-2、Bcl-xl和Mcl-1抗凋亡蛋白的表达,同时增加促凋亡蛋白Bax和C-PARP的表达。结论:DHA可显著抑制下咽鳞状细胞癌Fadu细胞的增殖,并明显增加细胞的凋亡,其机制与DHA调控Bcl-2家族密切相关。提示,Bcl-2家族成员在DHA抑制下咽鳞状细胞癌Fadu细胞增殖的过程中发挥了重要作用。

Objective：To investigate the effect of dihydroartemisinin （DHA） on the apoptosis of Fadu cells. Method：Fadu cells were treated with DMSO or different doses of DHA for 24 or 48 h. After that, IC50 values of DHA were calculated based on the cell viability tested via MTT assay, the cell morphology was observed,the percentage of apoptotic cells was analyzed with the Annexin V-FITC Apoptosis Detection Kit, and some important regulators of apoptosis,such as Bcl-2, Bcl-xl, Mcl-1, Bax, and C-PARP were determined by Western blotting. Result：Certain doses of DHA significantly inhibited the proliferation and induced apoptosis of Fadu cells in a dose time-dependent manners. DHA also downregulated the expression of antiapoptotic proteins （Bcl-2, Bcl-xl, and Mcl-1） and upregulated the expression of proapoptotic proteins（Bax and C-PARP）. Conclusion： DHA remarkably inhibited proliferation and induction of apoptosis in Fadu cells via affecting proteins of Bcl-2 family. DHA may be a promising drug to treat hypopharyngeal carcinoma.