口腔鳞癌肿瘤巨细胞与临床病理因素的相关性研究

Correlation of giant cancer cells in human oral squamous cell carcinoma with clinicopathological characteristics

目的:探究肿瘤巨细胞(giant cancer cell,GCC)在口腔鳞癌(oral squamous cell carcinoma,OSCC)组织中的存在情况及其与OSCC临床病理因素的关系。方法:回顾性分析2005年3月至2014年12月天津医科大学肿瘤医院76例OSCC患者的石蜡切片及临床病理资料。H&E染色及Ki-67免疫组织化学染色下观察GCC与普通肿瘤细胞的形态差异,计数GCC细胞,分析其与OSCC临床生物学行为及预后的关系。结果:H&E染色下观察到GCC存在于OSCC中,且Ki-67在GCC中均呈阳性表达。GCC细胞数与临床分期(P=0.02)、组织学分级(P<0.01)、化疗(P=0.01)及局部复发(P=0.02)均显著相关。GCC细胞数在不同性别和年龄的患者中差异不大,且与淋巴结转移、远处转移无关(P>0.05)。GCC数目>3个/HP的OSCC患者预后较差(P<0.05)。结论:GCC可能在OSCC发展过程及耐药中起重要作用,可能是影响OSCC预后的重要因素。

Objective：To detect giant cancer cells in human oral squamous cell carcinoma （OSCC） and elucidate their correlation with OSCC clinicopathological characteristics. Methods：Tumor paraffin sections and clinicopathological information of 76 OSCC cases were collected from Tianjin Medical University Cancer Institute and Hospital. Morphological differences between giant cancer cells and nor-mal-sized cancer cells were observed under H＆amp;E staining and Ki-67 immunohistochemistry staining, respectively. The number of giant cancer cells was counted under a microscope （×400）. The association between the number of giant cancer cells and the biological be-havior of OSCC was analyzed. Results：Giant cancer cells were observed in OSCC tissues, and these cells were positive for Ki-67 stain-ing. The number of giant cancer cells was closely associated with clinical stage （P=0.02）, histological grade （P〈0.01）, chemotherapy （P=0.01）, and local recurrence （P=0.02）. By contrast, the number of giant cancer cells was not associated with sex, age, and lymphatic and distant metastases in OSCC （P〉0.05）. Patients with more than 3/HP giant cancer cells retained poor prognosis （P〈0.05）. Conclusion：Gi-ant cancer cells may play an important role in the progression, chemotherapeutic resistance, and prognosis of OSCC.