多溴联苯醚的酶联免疫吸附分析法

Enzyme-linked Immunosorbent Assay for Determination of Polybrominated Diphenyl Ethers

为实现对多溴联苯醚(PBDEs)的快速检测,选取C_(15)H_9Br_3O_3和C11H13O3NBr2作为2,2′,4,4′-四溴联苯醚(BDE-47)的半抗原进行合成,并通过1H核磁共振波谱(1HNMR)对其进行结构鉴定,采用活性酯法使C_(15)H_9Br_3O_3与锁孔血蓝蛋白(KLH)和牛血清白蛋白(BSA)偶联合成免疫原,免疫动物后制备2种特异性抗血清,建立了关于多溴联苯醚类物质的间接竞争抑制酶联免疫吸附分析(ELISA)法。研究表明,C_(15)H_9Br_3O_3-KLH抗血清测定BDE-47的IC50值为0.60 mg/L,检出限为0.08 mg/L,C_(15)H_9Br_3O_3-BSA抗血清测定BDE-47的IC50值为0.11 mg/L,检出限为0.01 mg/L。建立的间接竞争抑制ELISA法可以满足对经过预处理后的环境样品中多溴联苯醚进行快速筛查的要求。

In order to achieve the rapid detection of polybrominated diphenyl ethers（PBDEs）, C_（15）H_9Br_3O_3 and C11H13Br2NO3ashaptens of 2, 2′, 4, 4′- tetra- BDE（BDE- 47） were synthesized,and their structural identifications were characterized by1 HNMR spec-troscopy. C_（15）H_9Br_3O_3 was conjugated to keyhole limpet hemocyanin（KLH） and bovine serum albumin（BSA） as immunogens by the activeester,respectively. Two kinds of antiserum were raised against the conjugate of C_（15）H_9Br_3O_3-KLH and C_（15）H_9Br_3O_3-BSA, which establishedindirect competitive inhibition enzyme-linked immunosorbent assay of polybrominated diphenyl ethers（ELISA） method. Studies haveshown that, the determination of IC50 value of C_（15）H_9Br_3O_3-KLH antiserum was 0.60 mg/L, and the detection limit was 0.08 mg/L, whilethe determination of IC50 value of C_（15）H_9Br_3O_3-BSA antiserum was 0.11 mg/L, and the detection limit was 0.01 mg/L. The established ELISAcan be used to rapid screening the PBDEs of environmental samples after preconcentration.