不同品种红掌试管苗生根体系优化研究

System optimization of rooting medium of the tube-shoots of different cultivars of Anthurium sp.

以红掌的试管苗为试验材料,研究了生根培养基的不同离子浓度(MS,1/2 MS,1/3 MS,1/5 MS)以及生根培养基中活性炭、NAA、IBA对其生根的影响,并对6个品种红掌(大哥大、粉冠军、火鹤、潘多拉、维多和阿瑞博)试管苗的生根效果进行了比较,结果发现:(1)1/2 MS培养基为促进红掌试管苗生根的最适基本培养基,在1/2 MS中加入2.0 g/L活性炭可显著提高植株生根率,降低黄化率;(2)IBA对根诱导效果优于NAA;两者均在质量浓度为0.000 2 g/L时诱导生根效果最好,质量浓度高于0.000 4 g/L时,诱导生根效果减弱;(3)试管苗以1/2 MS+2.0 g/L活性炭+0.000 2 g/L IBA生根培养,大哥大、潘多拉、粉冠军和阿瑞博4品种生根效果较好,在单株生根数和单株根长大于1 cm的根数2个指标上,显著好于火鹤、维多。

After the tube-thoots of Anthurium sp. were used as experimental materials to study the effect of the rooting medium with different ion concentrations,activated carbon,NAA and IBA,the rooting differences were researched on six cultivars Anthurium sp. shoots. The results showed that 1） 1 / 2 MS was the optimal basic ion concentration. And 1 / 2 MS medium adding 2.0 g / L activated carbon could significantly improve the rate of rooting and decrease the rate of yellowing; 2）The effect of IBA on rooting was better than that of NAA; 0.000 2 g / L IBA effected best but its effect weakened when higher than 0.000 4 g / L; 3） The rooting effects of ‘Dakota＇,‘Pink Champion＇,‘Pandora＇,‘Arebo＇of six cultivars of Anthurium sp. in 1 / 2 MS supplemented with 2. 0 g / L activated carbon and 0.000 2 g / L IBA were better than those of ‘A.scherzerianum＇and‘Vito＇. Also,the root number of per shoot and the number of root longer than 1 cm of ‘Dakota＇,‘Pink Champion＇,‘Pandora＇,‘Arebo＇were significantly more than those of‘A. scherzerianum＇and‘Vito＇.