羊蹄甲属5个树种花粉生活力测定

Measurement on Pollen Viability of Five Species from Bauhinia

采用MTT法和离体萌发法对红花羊蹄甲(Bauhinia blakeana)等5个羊蹄甲属树种花粉生活力进行测定,结果表明,红花羊蹄甲、黄花羊蹄甲(B.tomentosa)、宫粉羊蹄甲(B.variegata)、橙红羊蹄甲(B.galpinii)和羊蹄甲(B.purpurea)花粉MTT染色率分别为40.3%、79.8%、93.6%、95.7%、100%,羊蹄甲、宫粉羊蹄甲、橙红羊蹄甲花粉细胞生活力较其他两个树种要高;离体萌发试验测得以上5个树种的花粉萌发率差异很大,红花羊蹄甲、黄花羊蹄甲、宫粉羊蹄甲、橙红羊蹄甲和羊蹄甲花粉的最高萌发率分别为0、23.0%、92.1%、50.0%、80.3%,其中宫粉羊蹄甲花粉萌发率最高,羊蹄甲花粉次之,红花羊蹄甲花粉不萌发。以上5个树种中宫粉羊蹄甲及羊蹄甲的盛花期花粉可以作为人工授粉进行杂交育种较为理想的花粉来源。宫粉羊蹄甲花粉最适离体萌发培养基配方为蔗糖220 g/L+硼酸20 mg/L。

The pollen viability of the five species of Bauhinia was studied by MTT and invitro germination method. The result showed that the MTT staining rates of Bauhinia blakeana, B. tomentosa, B. variegata, B. galpinii and B. purpurea were 40.3%, 79.8%, 93.6%, 95.7% and 100%, respectively. The viability of pollen cell of B. purpurea, B. variegata and B. galpinii were higher than other two species. There were great differences in 5 tree species in the invitro germination test result, and the highest germination rate of B. blakeana, B. tomentosa, B. variegata, B. galpinii and B. purpurea were 0、23.0%、92.1%、50.0%、80.3%, respectively. The B. variegata pollen germination rate was the highest, followed by B. purpurea, and the pollen of B. blakeana was not germinated. In the five species, the flowering period pollen of B. variegata and B. galpinii can be an ideal pollen source for artificial breeding. The optimum medium for the invitro germination of B. variegata pollen was sucrose（220 g/L） and boric acid（20 mg/L）.