结缔组织生长因子在香烟提取物致人肺动脉平滑肌细胞增殖中的作用研究

EFFECTS OF CIGARETTE SMOKE EXTRACT ON PROLIFERATION AND CTGF EXPRESSION OF HUMAN PULMONARY ARTERY SMOOTH MUSCLE CELLS

目的探讨香烟提取物(CSE)对人肺动脉平滑肌细胞(hPASMC)增殖的影响及结缔组织生长因子(CTGF)在其中的作用。方法培养的hPASMC随机分为正常对照组(A)、缺氧对照组(B)、CSE组(C)、CSE+rhTGF-β1组(D)、CSE+CTGF正义寡核苷酸组(E)、CSE+CTGF反义寡核苷酸组(F),分别检测细胞增殖、CTGF mRNA及蛋白表达、细胞培养上清液中I型胶原含量。结果细胞增殖检测,C组与A组比较明显升高,D组与C组比较,D组升高更卓著,F较C组下降显著(P均<0.01);E和C组之间无显著性差异(P>0.05)。hPASMC内CTGF蛋白表达量C组较A组明显升高,D较C组升高更明显,F较C组下降显著(P均<0.01);E组与C组之间无显著性差异(P>0.05);CTGF mRNA表达结果及细胞培养上清液中I型胶原含量均与蛋白表达有相似趋势。结论 CSE能诱导hPASMC增殖,CTGF可能在其中起重要作用。

Objective In order to explor the effects of cigarette smoke extract （CSE） on cell proliferation and the role of connective tissue growth factor （CTGF）. Methods In this study, Cultured hPASMCs were divided into six groups： A： the normal control group, B：hypoxia control group, C：CSE group, D.CSE and rhTGF-β1 group, E：CSE and CTGF sense oligonucleotide group, F： CSE and CTGF antisense oligo- nucleotide group. Then, the expression of CTGF mRNA and protein of the supernatant of cells, collagencontent of cell culture supernatant. Results In Cell proliferation assay, C group was more increased than in group A respectively, Compared with D and C group, D group was more outstanding, F groupwas decreased significantly, compared with C group （P 〈 0. 01）. There was no significant difference between group E andC （P〉 0.05）. The results of CTGF mRNA ex- pression and the content of type Ⅷ collagen in the su-pernatant of cell culture were showed similar tendency to the protein expression. Conclusion CSE can in- duce hPASMC proliferation, CTGF may play an important role in it.