曲安奈德对棕色挪威大鼠脉络膜新生血管生长的影响

Effects of Triamcinolone Acetonide on Choroidal Neovascularization in Brown Norway Rats

目的观察光凝后即刻玻璃体腔内注射曲安奈德对棕色挪威大鼠脉络膜新生血管生长的影响。方法 36只健康棕色挪威大鼠随机分为对照组和实验组,每组18只,随机抽取一眼作为实验眼。建立棕色挪威大鼠脉络膜新生血管动物模型。视网膜光凝后,实验组立即玻璃体腔内注射曲安奈德8μl;对照组在实验眼玻璃体内注射同等容积的等渗平衡盐溶液。分别在光凝后2、4、6周取6只大鼠,采用免疫组化法检测核因子-κB蛋白的表达,测量脉络膜新生血管的最大面积。结果实验组的脉络膜新生血管面积及核因子-κB蛋白的表达低于同期对照组(t>7.450,P<0.001)。结论曲安奈德可抑制棕色挪威大鼠脉络膜新生血管的生长,降低核因子-κB蛋白的表达。

Objective To observe the effect of intravitreal triamcinolone acetonide injection on choroidal neovascularization in brown Norway rats. Methods Thirty-six healthy brown Norway rats were divided randomly into control group（n=18） and experimental group（n=18）, while one eye was chosen randomly as experimental eye. The choroidal neovascularization model was establishied, while 8 μl triamcinolone acetonide was injected in vitreous body immediately after photocoagulation in the experimental group, and the same volume isotonic balanced salt solution was injected in the control group. The eyes of six rats were enucleated for histological slices in the second, forth, and sixth week, respectively. The expression of nuclear factor- kappa B was detected with immunohistochemical method, and the max area of choroidal neovascularization was also measured. Results The area of choroidal neovascularization was smaller, and the expression of nuclear factor-kappa B was lower in the experimental group than in the control group（t〉7.450, P〈0.001）. Conclusion Triamcinolone acetonide could effectively inhibit the development of choroidal neovascularization, and decrease the expression of nuclear factor-kappa B in brown Norway rats.