芒果苷对四氧嘧啶糖尿病小鼠的降糖作用研究

Hypoglycemic Effects of Mangiferin in Alloxan-induced Diabetic Mice

本研究以二甲双胍为阳性对照,观察芒果苷(12.5、25.0、50.0 mg/kg)灌胃给药4周,对四氧嘧啶性糖尿病小鼠糖脂代谢的影响。实验结果表明芒果苷(25.0、50.0 mg/kg)灌胃给药后显著降低糖尿病小鼠的血糖、血清甘油三酯和总胆固醇水平,增加小鼠肌、肝糖原含量,但对糖尿病小鼠血清胰岛素水平没有影响;组织病理学检查结果表明芒果苷对糖尿病小鼠胰岛和β细胞的数量无明显改善,提示芒果苷灌胃给药对四氧嘧啶性糖尿病小鼠有降血糖作用,其降糖作用可能与促进肌、肝糖原的合成,增加机体对葡萄糖的利用有关,对糖尿病小鼠的脂代谢紊乱也有一定改善作用。

This study was carried out to investigate the antidiabetic activities and mechanism of mangiferin in alloxan-indueed diabetic mice. Adult male ICR mice were injected with a single dose of alloxan 60 mg/kg body weight though tail vein to induce diabetic animals. Mangiferin was administered intragastrically at a dose of 12.5,25.0 and 50.0 mg/kg to diabetic mice for four weeks. Mefformin treated diabetic mice were served as positive control. The levels of blood glucose were monitored weekly. At the end of experiment, blood glucose, serum insulin, triglyeeride and total cholesterol levels were determined, the glycogen contents of hepatic and skeleta/muscle were also measured. The pancreatic islet and β- cell were identified by staining with hematoxylin eosin （HE） and aldehyde-fuchsin. After 4 weeks administration, mangiferin （25.0,50, 0 mg/kg） significantly declined the levels of blood glucose, serum triglyeeride and total cholesterol, and increased the contents of muscle and hepatic glycogen, compared with the untreated diabetic mice. However, man- giferin was unable to significantly increase the level of serum insulin and the number of pancreatic islet and β-cell. These results suggested that mangiferin possessed antidiabetie activities in alloxan-induced diabetic mice. The mechanism was related to improve synthesis of muscle and hepatic glycogen,increase the body＇ s use of glucose. In addition, mangiferin also had the regulating effect on the disorder of lipid metabolism.