基于代谢组学的蒙药黄精保健功能的作用机制研究

Study on the mechanism of health protection of Mongolian medicine Polygonatum sibiricum based on metabonomics

目的利用代谢组学方法探究保健药黄精对机体的作用机制.方法将超高效液相色谱-质谱联用技术(UPLCMS)与主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)相结合,对大鼠以黄精灌胃后体内内源性物质的变化情况进行研究.结果在PCA与OPLS-DA模型中,正常组与给药组大鼠血液样本均可明显区分.大鼠给药后共获得6个生物标记物,分别α-生育三烯酚、13'-羧基-γ-生育酚、硬脂酸、胆固醇、溶血磷脂酰乙醇胺[0:0/18:2(9Z,12Z)]、溶血磷脂酰乙醇胺[0:0/20:4(8Z,11Z,14Z,17Z)],其中α-生育三烯酚、13'-羧基-γ-生育酚、硬脂酸的含量升高,而胆固醇、溶血磷脂酰乙醇胺[0:0/18:2(9Z,12Z)]、溶血磷脂酰乙醇胺[0:0/20:4(8Z,11Z,14Z,17Z)]的含量降低.结论蒙药玉竹具有保健作用的原因可能是由于其改变了血液中某些成分的含量。

Objective To explore the effects of Polygonatum sibiricum on body based on metabonomics. Methods The ultra per- formance liquid chromatography coupled with mass spectrometry （ UPLC - MS） and principle component analysis （PCA） and or- thogonal partial least squares discriminant analysis（ OPLS- DA） were used to evaluate the changes of endogenous substances in rats after intragastric administration of Polygonatum sibiricum. Results Significant differences between the control group and ad- ministration group were observed in PCA and OPLS - DA model. Six potential biomarkers between control group and administra- tion group were identified. The relative content of 13＇- carboxy - γ- tocotrienol, Aipha - Tocotrienol, Stearic acid increased, then the relative content of Polyporusterone C, LysoPE （ 0 ： 0//18 ： 2 （ 9 Z, 12Z ） ）, LysoPE （ 0 ： 0/20 ： 4 （ 8 Z, 11 Z, 14Z, 17 Z ） ） decreased. Conclusion The reason why the Mongolian medicine Polygonatum sibiricum has the function of health care may be due to the change of the content of some components in the blood.