摘要
目的研究资木瓜乙酸乙酯萃取物对不同抗原诱导的RBL-2H3细胞脱颗粒的影响,并初步探讨其作用机制。方法采用MTT法测定不同浓度资木瓜乙酸乙酯萃取物对细胞活性的影响,采用底物法检测不同浓度资木瓜乙酸乙酯萃取物对不同抗原引起的细胞内β-氨基己糖苷酶释放的影响,采用流式细胞分析仪检测不同浓度资木瓜乙酸乙酯萃取物对细胞表面受体FcεRIα表达的影响。结果在一定浓度范围内,对细胞活性没有显著性影响的资木瓜乙酸乙酯萃取物可显著抑制细胞内β-氨基己糖苷酶的释放和降低细胞表面受体FcεRIα的表达,且呈量效关系。结论资木瓜乙酸乙酯萃取物对RBL-2H3细胞脱颗粒具有显著的抑制作用,其作用可能与RBL-2H3细胞表面受体FcεRIα的表达降低有关。
Objective To investigate the influence of Chaertomeles speciosa ethyl acetate extract on the degranulation of rat baso- philic leukemia cells （RBL -2H3 ） and to research the possible mechanism. Methods The effect of different density of Chaenomeles speciosa ethyl acetate on the cell viability of RBL - 2H3 cells by MTT assay; The effect of different density of Chaenomeles speciosa ethyl acetate on the releasing rate of β - hexosaminidase enzyme of RBL - 2H3 cells by Substrated method ; The effect of different density of Chaenomeles speciosa ethyl acetate on the surface expression of receptor FcεRIα of RBL - 2H3 cells by Flow cytometry. Results Chaenomeles speciosa ethyl acetate extract has no significant effect on cell activity in some densi- ty, but it can significantly decrease the release of β - hexosaminidase and the expression of cell surface receptor FcεRIα. Con- clusion Chaenomeles speciosa ethyl acetate extract can inhibit the degranulation of RBL - 2H3 cells in a dose - dependent manner and the mechanism may related to the down - regulated expression of cell surface receptor FcεRIα.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2016年第10期2356-2358,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.81274166)
