气营传变模型兔舌背黏膜TLR4和E-cad表达研究

The expression of TLR4 and E-cad of the tongue dorsum mucous membrane in rabbit models of the transformation from Qifen to Yingfen

目的探讨气营传变对兔舌背黏膜TLR4(Toll like receptors 4,Toll样受体4)和E-cad(E-cadherin,E-钙粘蛋白)表达的影响。方法利用LPS(Lipopolysaccharide,脂多糖)对兔耳缘静脉进行注射,建立气分证和营分证模型,以白虎汤和清营汤对证干预。HE染色及免疫组化研究TLR4和E-cad表达水平。结果与对照组相比,营分证组舌背黏膜显著增厚(P<0.01),TLR4在气分证与营分证组中的表达均显著升高(P<0.01),气分证及营分证组中舌背黏膜E-cad表达显著降低(P<0.01),清营汤可显著逆转营分证组舌背黏膜TLR4表达(P<0.05)和E-cad表达(P<0.01),而白虎汤效果不显著(P>0.05)。结论气营传变过程影响兔舌背黏膜厚度,与TLR4、E-cad表达变化有关。

Objective To investigate the expression of Toll like receptors4 （ TLR4 ） , E - cadherin （ E - cad） of the transforma- tion from Qifen to Yingfen on changes of tongue dorsum. Methods Lipopolysaccharide （LPS） was injected to the rabbits by au- ricular vein to establish the model of the Qifen Syndrome and Yingfen Syndrome. The treatment of Baihu Tang on the Qifen Syn- drome and Qingying Tang on the Yingfen Syndrome. HE staining and immunohistoehemistry were used to obsmwe the expression of TLR4 and E - cad . Results Compared with the control group, the tongue mucosal thickness of Yingfen Syndrome group was thic- ker （P 〈 0.01 ）. E -cad in both Qifen Syndrome group and Yingfen Syndrome group was markedly lower （P 〈 0.01 ）. Qingying Tang could obviously reverse the expression of the E - cad （ P 〈 0.01 ） and TLR4 （ P 〈 0.05 ） on the tongue dorsum mueosal membrane of the Yingfen Syndrome while the effect of Baihu Tang was not obviously （ P 〉 0.05 ）. Conclusion TLR4 and E - cad are signifieance molecule to reflect the changes of tongue during the transformation from Qifen to Yingfen.