SQA-V精子质量分析仪与精子形态学分析在精液检测中的应用

Application of SQA-V sperm quality analyzer and sperm morphologic assessment on seminal fluid detection

目的分析绵阳地区不育男性精液质量现状,为该地区男性不育症的诊断、治疗和疗效观察提供依据。方法采用SQA-V全自动精液质量分析仪与精子形态学分析检测1 773例不育男性的精液标本,并对结果进行分析。结果在1 773例检测标本中,精液量<1.5 ml者占137例(7.73%),液化时间>60 min占529例(29.84%),精子密度<15×106/ml占255例(14.38%),精子活力a+b级<32%占463例(26.11%),活动率<50%占584例(32.94%)。男性不育组中,无精子症(2.20%)、少精子症(9.70%)、弱精子症(22.90%)、少弱精子症(4.23%)以及严重少弱精子症(0.62%)患者比例均高于对照组,差异有统计学意义(P<0.05)。男性不育患者精液正常形态精子百分率<15%者有1 568例,高达88.44%。结论将精液常规与精子形态学相结合,客观正确的分析精液结果,对判断男性生育能力有重要意义。

Objective In order to provide a scientific basis for diagnosis and clinical treatment of male infertility and improve treatment efficacy, the semen quality of infertile men in Mianyang areas was investigated. Methods 1 773 semen specimens of infertile men were collected. Seminal fluid detection was performed by SQA-V automatic sperm quality analyzer and detection of sperm morphology. The results were statistically analyzed. Results In the 1 773 semen samples, there were 137 cases （7.73%） with semen volume less than 1.5 ml, 529 cases （29.84%） with liquefaction time more than 60 min, 255 cases （14.38%） with sperm concentration less than 15 ×10^6 /ml, 463 cases （26.11%） with a and b grade sperm motility less than 32%, and 584 cases （32.94%） with activity rate less than 50%. Above all abnormalities account for a large percentage. The proportions of azoospermia （2.20%）, oligozoospermia （9.70%）, asthenospermia （22.90%）, oligoasthenospermia （4.23%） and severe oligoasthenospermia （0.62%） in the infertility group were higher than those in the control group. The differences were statistically significant. In male infertility group, there were 1 568 semen （88.44%） with normal sperm morphologic features less than 15%. Conclusion Sperm routine analysis combined with sperm morphologic assessment could objectively and correctly evaluate male fertility.