Bcl-3在人乳腺癌组织中的表达及对乳腺癌MDA-MB-231细胞增殖与凋亡的影响

Expression of Bcl-3 in breast cancer tissues and its effect on the proliferation and apoptosis of human breast cancer cell line MDA-MB-231

目的:探讨Bcl-3在乳腺癌中的表达及沉默后对乳腺癌细胞生物学行为的影响。方法:选取80例乳腺癌组织及相应癌旁组织,采用免疫组织化学法检测其中Bcl-3蛋白表达,并分析癌组织中Bcl-3表达与临床病理参数间的关系;将乳腺癌MDA-MB-231细胞随机分为空白对照组(常规培养),阴性对照组(转染对照si RNA)和Bcl-3 si RNA组(转染Bcl-3 si RNA)。转染48 h后,免疫印迹法检测各组Bcl-3表达;MTT、平板克隆实验及流式细胞术分别检测各组细胞增殖能力、克隆形成能力和细胞凋亡。结果:Bcl-3蛋白在乳腺癌及相应癌旁组织中阳性表达率分别为72.5%和51.3%。乳腺癌组织中Bcl-3蛋白表达与组织学分级、淋巴结转移及Her-2表达呈正相关(P<0.05),而与年龄、肿瘤大小、病理分型及雌、孕激素受体无关(P>0.05)。空白对照组和阴性对照组Bcl-3表达水平明显高于Bcl-3 si RNA组(P均<0.01)。与空白对照组和阴性对照组比较,Bcl-3 si RNA组细胞增殖率和克隆形成率降低,细胞凋亡率增高(P均<0.01)。结论:Bcl-3在乳腺癌组织中呈高表达,其沉默后起抑癌作用,乳腺癌细胞增殖能力及克隆形成率降低、凋亡率增高。

Objective：To explore Bcl-3 expression in human breast cancer and its effect on breast cell proliferation and apoptosis.Methods：Bcl-3 expression in 80 breast cancer tissues with corresponding adja-cent tissues were detected by immunohistochemical staining.MDA-MB-231 cells were randomized into blank control group（routine culture）,negative control group（transfected with control siRNA）and Bcl-3 siRNA group（transfected with Bcl-3 siRNA）;Bcl-3 expression was detected by western blotting.MTT as-say,plate clone formation assay and flow cytometry were used to determine the cell proliferation,cloning formation efficiency and apoptosis rate,respectively.Results：The positive rate of Bcl-3 expression in breast cancer tissue was 72.5%,while in adjacent tissues was 51 .3%.The Bcl-3 expression in breast cancer tissues was positively correlated with histological grade,lymph node metastasis and Her-2 expression （P〈0.05 ）.Bcl-3 expression in blank control group and negative control group was significantly higher than in Bcl-3 siRNA group（P〈0.01 ）.Compared with blank control group and negative control group,Bcl-3 siRNA group showed lower cell proliferation rate,lower colony forming efficiency,higher apoptosis rate （P〈0.01 ）.Conclusion：Bcl-3 expression in breast cancer tissues was high,and silencing it could suppress the growth of MDA-MB-231 breast cancer cells by reducing cell proliferation,inhibiting cloning formation,and inducing cell apoptosis.