四君子汤复方多糖对脾虚小鼠血清代谢组学的影响

Effect of Sijunzi Decoction Polysaccharide on Metabolomics of Spleen Deficiency Mice Serum

目的 :探讨四君子汤复方多糖对脾虚小鼠血清代谢组学的影响。方法 :运用液-质联用(LC-MS)技术采集血清中内源性物质数据,用偏最小二乘判别分析(PLS-DA)方法对试验数据进行分析,寻找四君子汤复方多糖干预的脾虚小鼠血清内源性标记物,阐述其作用机制。结果:PLS-DA得分图中四君子汤复方多糖治疗组与脾虚模型组分离良好,多糖组内源性标记物含量有向正常组靠近的趋势,表明四君子汤复方多糖对脾虚小鼠有一定的改善作用。结论:通过代谢组学技术,确定了四君子汤多糖干预脾虚小鼠的内源性标记物,这些潜在的标记物代谢通路有待于进一步研究。

Objective：To investigate the effect of Sijunzi decoction polysaccharide on metabolomics of spleen deficiency mice serum. Methods：The endogenous material data in serum were acquired based on liquid chro matography coupled to quadrupole time of flight mass spectrometer（LC-MS）. The metabolic data were an- alyzed with partial least square discriminant analysis（PLS-DA） to determine serum endogenous markers of spleen deficient mice intervened with Sijunz~ decoction polysaccharide and to explore the mechanism. Re- suits：In the PLS-DA score plot,Sijunzi decoction polysaccharide treatment group and the spleen deficient model group were clearly distinguished,and in the polysac＇charide group endogenous biomarkers content tend- ed to be normal,indicating Sijunzi decoction polysaccharide had certain effect on the spleen deficiency mice. Conclusion：Through metabonomics,the endogenous marker of Sijunzi decoction polysaccharide intervening spleen deficient mice were determined. Metabolic pathways of these potential markers remains to be further studied.