温度对Bt水稻及其非靶标害虫褐飞虱生态适应性的影响

Effects of temperature on Bt rice and ecological fitness of the non-target insect pest Nilaparvata lugens

【背景】温度是影响植物生长发育最为重要的环境因子之一。明确温度对Bt水稻及其非靶标害虫的影响有助于今后Bt水稻的推广应用。【方法】设置不同的温度梯度(26、30和34℃)分别处理Bt水稻T2A-1(表达Cry2A蛋白),在处理后不同天数内分别测定Cry蛋白、叶绿素、草酸、可溶性糖和总氮含量,同时评价不同温度处理下Bt水稻上褐飞虱的生态适应性。【结果】Cry蛋白含量显著受温度影响,随着温度升高Cry蛋白含量下降。温度、处理时间及两者相互作用均显著影响Bt水稻T2A-1的叶片叶绿素、叶鞘草酸、可溶性糖和总氮含量。叶绿素含量在26℃处理20 d时高于30、34℃的处理。随着温度的升高,Bt水稻叶鞘草酸含量升高。可溶性糖含量高温(30和34℃)处理后20 d低于26℃处理。Bt水稻叶鞘内可溶性氮含量随着温度的升高而升高。温度对非Bt水稻MH63上叶绿素、草酸、可溶性糖和总氮含量的影响与T2A-1趋势一致。高温(34℃)处理的Bt水稻上的褐飞虱雌雄虫寿命、每雌产卵量及卵孵化率显著低于26℃处理。温度对非Bt水稻MH63上褐飞虱生态适应性参数的影响与T2A-1趋势一致。【结论与意义】温度对Bt水稻具有显著影响,且对Bt水稻上非靶标害虫褐飞虱的生态适应性产生不利影响。本研究有利于今后Bt稻田的害虫综合治理措施的制定。

【 Background】 Temperature is one of the most important factors that influences the growth and development of crop plants. Understanding the effects of temperature on Bt rice and its non-target insects is helpful for the implement and management of Bt rice in future.【 Method】 The contents of Cry protein,chlorophyll,oxalic acid, soluble sugar and nitrogen were investigated in the Bt rice plants that were exposed to different temperatures （ 26,30 and 34 ℃）for different durations. Ecological fitness of brown plan- thopper Nilaparvata lugens was evaluated under these different temperatures.【 Result】 The Cry protein content was significantly af-fected with decreasing Cry protein as the temperature increased. Temperature,treatment duration and their interaction were signifi-cantly for the content of chlorophyll,oxalic acid,soluble sugar and nitrogen in Bt rice. The content of chlorophyll in Bt rice and sol-uble sugar of Bt rice shezth treated for 20 d at 26 ℃ was higher than at 30 and 34 ℃. The content of oxalic acid and nitrogen in Bt rice sheath increased with increasing temperatures. The effect of temperature on the chlorophyll,oxalic acid,soluble sugar and nitro-gen in non-Bt rice MH63 were similar to T2A-1. Longevity of male and female adults,number of eggs deposited per female and egg hatchability of N. lugens on Bt rice was lower at 34 ℃ than at 26 The effect of temperature on the ecological fitness of the nontar-get insect pest N. lugens in non-Bt rice MH63 was similar to T2A -1 . 【Conclusion and significance 】 Temperature could influence the physiology of Bt rice and negatively affect the ecological fitness of the nontarget insect pest N. lugens in Bt rice. This study will facili-tate the makeup of the integrated management of insect pests in the Bt rice in future.