MCRS1、MCRS2基因在结直肠癌中的表达研究

Expression of MCRS1 and MCRS2 in colorectal cancer and its clinical significance

目的检测微球蛋白MCRS1、MCRS2基因在结直肠癌(CRC)中的表达情况,探讨其与临床指标的相关性及临床意义。方法选取32例CRC患者手术切除的肿瘤组织和癌旁正常组织为研究材料,RT-PCR检测MCRS1、MCRS2基因在肿瘤、癌旁正常组织中的表达水平,分析基因表达水平与多项临床指标间的相关性。结果 (1)MCRS1基因在肿瘤组织中的表达阳性率高于在癌旁正常组织中的表达阳性率(93.75%vs 68.75%,P<0.05),而MCRS2基因在肿瘤组织中的表达阳性率低于在癌旁正常组织中的表达阳性率(71.88%vs 93.75%,P<0.05)。(2)MCRS1基因在肿瘤组织中的表达水平高于在癌旁正常组织中的表达水平(P<0.05),且其在晚期肿瘤组织内的表达水平较在早期肿瘤组织内表达水平高(P<0.05),其在侵透外膜的肿瘤组织内的表达水平也高于未侵及外膜者(P<0.05)。(3)MCRS2基因在肿瘤组织中的表达水平低于在癌旁正常组织中的表达水平(P<0.05),且其在晚期肿瘤组织内的表达水平较在早期肿瘤组织内表达水平低(P<0.05),其在侵透外膜的肿瘤组织内的表达水平也低于未侵及外膜者(P<0.05)。(4)MCRS1、MCRS2基因在肿瘤组织中的表达水平呈负相关(r=-0.4656,P<0.05),MCRS1基因在肿瘤组织中的表达水平与患者血清癌胚抗原(CEA)水平呈正相关(r=0.5045,P<0.05),而MCRS2基因在肿瘤组织中的表达水平与血清CEA水平无明显相关性(r=-0.0139,P>0.05)。结论 MCRS1、MCRS2基因在CRC肿瘤组织中的表达水平显著不同,MCRS1基因可能是促癌基因,而MCRS2基因可能是抑癌基因。

Objective To investigate the expression of microspherule protein 1 （MCRS1） and microspherule protein 2（MCRS2）in colorectal cancer and its clinical significance. Methods The expression levels of MCRS1 and MCRS2 mRNA were detected with RT-PCR in 32 samples of colorectal cancer （CRC）and corresponding adjacent normal tussue. The association of MCRS1 and MCRS2 expression with clinical indicators of patients was analyzed. Results The positive rate of MCRS1 expression in CRC was higher than that in normal tissues （93.75% vs 68.75%, P〈0.05）, but MCRS2 expression was lower in CRC than that in normal tissue（71.88 %vs 93.75%, P〈0.05）. The expression of MCRS1 in advanced CRC was higher than that in earlier one（P〈0.05） and the expression in CRC with serosal invasion was higher than that in CRC without serosal invasion（P〈0.05）.The expression of MCRS1 was negatively correlated with that of MCRS2（r=-0.4656, P〈0.05）. Serum CEA levels were positively correlated with the expression levels of MCRS1, but not with expression of MCRS2（r=-0.0139, P 〉0.05）. Conclusion The expression of MCRS1 is up-regulated and the expression of MCRS2 is down-regulated in CRC tissues, suggesting that MCRS1 may bea cancer-promoting gene, while MCRS2 may be a tumor suppressor gene.