螃蟹脚全粉的急性毒性和遗传毒性

Acute and Genetic Toxicity of Whole Powder of Viscum liquidambaricolum Hayata

参考2015年实施的GB 15193.1—2014《食品安全性毒理学评价程序》的实验方法,采用急性毒性实验、骨髓嗜多染红细胞微核实验、精原细胞染色体畸变实验和Ames实验,观察螃蟹脚全粉的急性毒性和遗传毒性作用。结果:螃蟹脚全粉对雌、雄性大鼠经口半数致死量(medium lethal dose,LD_(50))均为10.25 g/kg,属实际无毒级;骨髓嗜多染红细胞微核实验和Ames实验结果均为阴性;精原细胞染色体畸变实验表明,10.25 g/kg和5.12 g/kg的螃蟹脚全粉可能引起染色体一过性的畸变率升高,2.57 g/kg未观察到该现象。结论:螃蟹脚全粉未显示存在急性毒性,未观察到明确的遗传毒性。

The acute and genetic toxicity of whole powder of Viscum liquidambaricolum Hayata were evaluated using acute toxicity, bone marrow polychromatic erythrocyte micronucleus, mouse spermatogonium chromosome aberration and Ames tests as described in the Chinese national standard Toxicological Evaluation Procedure for Food Safety（GB 15193.1-2014）. The oral acute toxicity test revealed that the medium lethal dose（LD_（50）） of the powder was 10.25 g/kg for both male and female rats, which suggested that it was actually non-toxic. Both bone marrow polychromatic erythrocyte micronucleus and Ames tests gave negative results. Spermatogonium chromosome aberration tests at 10.25 and 5.12 g/kg doses revealed temporarily high aberration rate, which was not observed in the 2.57 g/kg group. Therefore, whole powder of Viscum liquidambaricolum Hayata showed neither acute nor genetic toxicity.