虫茶多酚对乙酰氨基酚致小鼠肝损伤的预防效果（英文）

Preventive Effects of Polyphenols from Insect Tea on Acetaminophen-Induced Hepatic Injury in ICR Mice

研究虫茶粗多酚(crude polyphenols of Insect tea,CPIT)对乙酰氨基酚致小鼠急性肝损伤的预防效果。在诱导肝损伤后,CPIT可以增加小鼠的体质量和减小肝脏质量,降低肝指数。经过CPIT处理后的小鼠较对照组小鼠(肝损伤)血清谷丙转氨酶、谷草转氨酶、乳酸脱氢酶、甘油三酯、血尿素氮含量均下降,而总胆固醇,白蛋白水平则上升。同时经过CPIT处理后,白细胞介素(interleukin,IL)-6、IL-12、肿瘤坏死因子-α和干扰素-γ的水平也较对照组小鼠降低。CPIT还可以上调肿瘤坏死因子-α、锰超氧化物歧化酶、铜锌超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶m RNA的表达和下调核转录因子(nuclear factor-κB,NF-κB)、一氧化氮合酶异型、环氧化酶的表达。通过结果可以看出,CPIT具有预防动物体内肝损伤效果。

The purpose of this study was to investigate the preventive effects of crude polyphenols from Insect tea（CPIT） on hepatic damage induced by acetaminophen in ICR mice. CPIT could increase body weight and reduce liver weight and liver index after induction of liver injury in mice. Serum levels of alanine aminotransferase, aspartate aminotransferase, lacate dehydrogenase, triglycerides, and blood urea nitrogen were decreased, while total cholesterol and albumin were increased in mice treated with CPIT as compared with liver injury controls. CPIT also resulted in a reduction in IL（interleukin）-6, IL-12, TNF（tumor necrosis factor）-α and IFN（interferon）-γ in comparison with control mice. It could increase the m RNA expression of IκB-α, Mn-SOD, Cu/Zn-SOD, CAT and GSH-Px, and decrease the m RNA expression of NF-κB, iNOS and COX-2. From the above findings, we can conclude that CPIT has a preventive effect on hepatic injury in vivo.