复方葛根片对衰老大鼠胸主动脉损伤的保护作用

Protective effect of compound puerarin tablet on thoracic aorta injury of aged rats

为研究复方葛根片对D-半乳糖致大鼠衰老后血清中同型半胱氨酸(Hcy)水平的影响及胸主动脉损伤的保护作用,将大鼠分为模型组,正常组,复方葛根片高、中、低剂量组。采用皮下注射D-半乳糖的方法制备大鼠衰老模型,运用AU5800全自动生化仪测定血清中Hcy的含量,运用SPSS16分析各Hcy含量和体重差异。对大鼠胸主动脉进行HE染色,在光镜下观察血管的组织病理学病变。研究结果表明,模型组与正常组以及各给药组与模型组的Hcy水平、体重均存在显著性差异(P<0.01))。复方葛根片各剂量组对胸主动脉血管具有不同程度的保护作用,高剂量组的保护作用最明显。研究认为复方葛根片能降低D-半乳糖致衰老大鼠血清中Hcy水平,并对胸主动脉的血管损伤具有保护作用。

We divided rats into model group and normal group, and high, middle and low dose group of compound puerarin tablet to investigate the impact of compound puerarin tablet on D-galactose induced serum homocysteine （Hcy） level and protective effect on thoracic aorta injury of aged rats. We employed subcutaneous D-galactose injection to establish rats aging model, AU5800 automatic biochemical analyzer to detect Hcy content in rats serum, and SPSS16 to analyze Hcy content and weight difference of each group. We also performed HE staining to rats thoracic aorta and observed its pathological changes with a light microscope. Results show that significant difference （P〈0.01） exists in Hcy level and weight of model group and normal groupl each dose group and model group. Different dose groups have different protective effects on thoracic aortic vessels, especially for the high dose group having best protective effect. We discover that compound puerarin tablets can reduce Hcy levels in D-galactose induced old rats serum and have protective effects on vascular injury of thoracic aorta.