摘要
The two-component regulatory system plays an important role in the growth and virulence of bacteria. In this study, the phosphate transcriptional regulatory protein （KdpE） gene of Vibrio alginolyticus strain HY9901 was cloned. Sequence analysis revealed that the length ofkdpE gene is 732 bp and encodes a putative protein of 243 amino acids. The predicted molecular weight （MW） of KdpE was 27.66 kD with an estimated pI of 5.01. Using SignalP 4.0 and TM- HMM Server 2.0 software, it was predicted that the KdpE protein was located in cytoplasm. It did not contain a signal peptide or a transmembranous region. A phylogenetic tree was constructed by MEGA 5.0 software, indicating that the KdpE of V. alginolyticus bad high genetic relationship with Vibrio campbellii and Vibrio parahaemolyticus. Using SWISS-MODEL work-space, the three-dimensional structures of conserved domain REC in the KdpE was determined. These results can provide a basis for further studies on the two-component regulatory systems of V. alginolyticus.
The two-component regulatory system plays an important role in the growth and virulence of bacteria. In this study, the phosphate transcriptional regulatory protein （KdpE） gene of Vibrio alginolyticus strain HY9901 was cloned. Sequence analysis revealed that the length ofkdpE gene is 732 bp and encodes a putative protein of 243 amino acids. The predicted molecular weight （MW） of KdpE was 27.66 kD with an estimated pI of 5.01. Using SignalP 4.0 and TM- HMM Server 2.0 software, it was predicted that the KdpE protein was located in cytoplasm. It did not contain a signal peptide or a transmembranous region. A phylogenetic tree was constructed by MEGA 5.0 software, indicating that the KdpE of V. alginolyticus bad high genetic relationship with Vibrio campbellii and Vibrio parahaemolyticus. Using SWISS-MODEL work-space, the three-dimensional structures of conserved domain REC in the KdpE was determined. These results can provide a basis for further studies on the two-component regulatory systems of V. alginolyticus.
基金
National Natural Science Foundation of China(31402344,31572656)
College Student Innovation Training Program(201510566042)
Major Program for the Fundamental Research of the Department of Education of Guangdong Province(2014GKXM046)
International Cooperation Innovation Platform Project of Universities in Guangdong Province(2013gjhz0008)
