银桔利咽含片中绿原酸、异嗪皮啶、迷迭香酸和苦玄参苷IA的HPLC波长切换法测定

Determination of Chlorogenic Acid,Isofraxidin,Rosmarinic Acid and Picfeltarraenin IA in Yinjie Liyan Hanpian by Wavelength Switching-HPLC

建立了高效液相色谱．波长切换法测定银桔利咽含片中的绿原酸、异嗪皮啶、迷迭香酸和苦玄参苷IA。采用AgilentTC-C18色谱柱，以乙腈（A）：0．2％磷酸（B）为流动相，梯度洗脱，切换波长检溅327nm（0～12min）测定绿原酸、342nm（12～25min）测定异嗪皮啶和迷迭香酸、264nm（25～50min）测定苦玄参苷IA。这4个化合物分别在6．0～108．0、1．0～18．0、1．4～24．5和0．9～16．6μg／ml浓度范围内线性关系良好，平均回收率分别为99．85％、99．31％、99．46％和99．30％，RSD分别为1．4％、1．6％、1．1％和1．4％。

An HPLC method combined with wavelength switching technique was established for the determination of chlorogenic acid, isofraxidin, rosmarinic acid and picfeltarraenin IA in Yinjie Liyan Hanpian. An Agilent TC-C18 column was used, with the mobile phase of acetonitrile （A） ： 0.2 % phosphoric acid （B） by gradient elution, at the switching detection wavelength of 327 nm （0-12 min） for chlorogenic acid, 342 nm （12--25 min） for isofraxidin and rosmarinic acid, 264 nm （25-50 min） for picfeltarraenin IA. The method was found to be linear for the above four compounds in the ranges of 6.0-108.0, 1.0-18.0, 1.424.5 and 0.9-16.6 μg/ml, respectively. Their average recoveries were 99.85%, 99.31%, 99.46% and 99.30%, with RSDs of 1.4%, 1.6%, 1.1% and 1.4%, respectively.