高速逆流色谱法分离纯化新疆圆柏枝叶中的黄酮类成分

Separation and purification of flavonoids from Juniperus sabina L.by high-speed counter-current chromatography

目的:建立高速逆流色谱分离纯化新疆圆柏枝叶中的黄酮类成分的方法。方法:以氯仿∶甲醇∶正丁醇∶水(4∶3∶0.5∶2)为溶剂系统,上相为固定相,下相为流动相,在流速2.0 m L/min、转速850 r/min、检测波长254 nm的条件下,对新疆圆柏中的黄酮类成分进行分离纯化。结果:从新疆圆柏提取物中分离纯化得到3个化合物:异槲皮苷(a,91.89%)、槲皮苷(b,98.45%)、槲皮素-3-O-(6″-O-乙酰基)-β-D-吡喃葡萄糖苷(c,95.35%),其中c为首次从圆柏属植物中分离得到的化合物。结论:该法快速简便,能够高效分离新疆圆柏中的黄酮类成分。

Objective：To study separation and purification of flavonoids from the leaves of Juniperus sabina L.by high -speed counter-current chromatography（ HSCCC）.Methods：Solvent system of chloroform： methanol： n-butanol： water（4： 3： 0.5： 2）was employed,the upper phase as stationary phase,the lower phase as mobile phase,a flow- rate of 2.0 mL/min, the rotation speed 850 r/min and detection wavelength 254 nm, flavonoids from Juniperus sabina Lwere isolated and purified under this condition.Results.Three compounds were separated from the leaves of Juniperus sabina L.as isoquercitrin（ a,91.89% ）, quercitrin（ b,98.45% ）, quercetin-3- O- （6＂- O-acetyl） -β-D- glucopyranoside（c, 95.35%）, of which compound c was separated from Juniperus genus for the first time. Conclusion：This method could conveniently and effectively separate flavonoids from Juniperus sabina L.