哮喘患儿血清IL-5、ECP的测定及相关性研究

Study on the dynamic changes of the serum interleukin 5(IL-5)and eosinophile cationic protein(ECP) level and their clinical value in children with asthma

为了探讨白介素_5(IL_5)、嗜酸粒细胞阳离子蛋白 (ECP)在儿童支气管哮喘发病机制中的作用 ,并评价其反映哮喘气道炎症的临床价值 ,收集23例哮喘急性发作患儿、22例哮喘缓解期患儿及17例正常儿童的外周血标本 ,用ELISA法测定血清IL_5的水平 ;以PharmaciaCAP系统检测血清中ECP的含量。结果 :哮喘急性发作组血清中IL_5、ECP水平明显高于缓解期组和正常组 ;且IL_5、ECP水平的升高程度与病情严重程度相关 ,两者之间呈正相关 ;而3组EOS计数差异无显著性。提示 :监测血清IL_5、ECP的动态变化 ,可以较好地反映气道嗜酸粒细胞炎症的反应过程 。

Asthma is a chronic inflammatory disease involved different kinds of the inlfammatory cells.Of them,eosinophiles and T-lymphocytes are the pivotal responding cells.In order to further investigate the role played by the eosinophiles and T-lymphocytes in the pathogenesis of the asthma,and evaluate their clinical value of the dynamic changes of the serum IL-5 and ECP level.So the serum IL-5 and ECP level in children with asthma were determined.The peripheral blood samples were collected from 62 children.Of them,23 cases were in the acute episode (boys 13 cases,girls 10 cases,age ranged from 3 to 15 years old,the mean age was 7.1 years old).22 cases were in the remission stage of the asthma(boys 13 cases,girls 9 cases,age ranged from 3-15 years old,mean age was 6.9 years old).The diagnostic criteria of the asthmas coincided with the diagnostic criteria of asthma stipulated by the national childhood asthma collaboration study group in 1988,with 17 normal children as the control group(boys 8 cases,girls 9 cases,age ranged from 4-13 years old,the mean age 8.7 years old).Serum IL-5 level was assayed with ELISA method,while the level of serum ECP was determined with phar-macia CAP system.The absolute eosinophile count(EOS)in peripheral blood was counted according to the routine examination.The serum level of ECP in acute episode group was significantly higher than that in remission stage and normal control group (P<0.05,<0.01 respectively).But there was no significant difference in comparison with the later 2 group(P>0.05).The serum IL-5 level in acute stage was markedly higher than that in remission stage and normal control group(P<0.001) with no significant difference in later 2 groups(P>0.05).The increasing degree of the serum IL-5 and ECP was closely associated with the severity of the disease with positive relationship between them.But no significant difference in EOS counts in these 3 groups.IL-5 is an important chemotaxis factor in the pathogenesis of the asthma.Mediating the eosinophile inflammation in the respiratory tract through eosinophile chemotaxis and activation,while ECP is the main inflammatory mediator released from the activated and degranulated eosinophile.It is one of the important indicators reflecting the development of the respiratory inflammation in children with asthma.So monitoring the dynamic changes of the serum IL-5 and ECP can correctly reflect the responding process of the eosinophile inflammation in the respiratory tract with the significant clinical value.