黄芩苷对牙周炎大鼠牙龈组织及外周血中IL-8表达的影响

Effects of baicalin on the expression levels of interleukin-8 in gingival tissues and peripheral blood of rats with periodontitis

目的采用大肠杆菌脂多糖诱导大鼠牙周炎模型,观察黄芩苷对牙周炎大鼠牙龈组织及外周血中白介素-8(IL-8)表达水平的影响。方法选用50只8周龄SD大鼠,随机分成5组,每组10只,A组为正常对照组,B组为牙周炎组,C1、C2、C3组为黄芩苷治疗组(分别以0.01、0.1、1.0μg/ml黄芩苷治疗),治疗1周后处死大鼠采取其外周血及牙龈组织,采用微量样本多重蛋白定量技术(CBA)检测外周血中IL-8表达含量,采用酶联免疫法(ELISA)检测牙龈组织中IL-8的表达含量。结果牙周炎组大鼠牙龈组织及外周血中IL-8表达含量均明显高于正常对照组(P<0.05),黄芩苷治疗组大鼠牙龈组织中IL-8和外周血中IL-8表达含量均明显低于牙周炎组(P<0.05),且C1、C2、C3组IL-8水平逐渐下降(P<0.05)。结论黄芩苷可以降低牙周炎大鼠牙龈组织和外周血中IL-8的表达含量,抑制脂多糖对大鼠牙周组织的损伤作用。

Objective To observe the effects of baicalin on the expression levels of interleukin-8 in gingival tissues and peripheral blood of rats with periodontitis induced by colibacillus lipopolysaccharide （LPS）.Methods Fifty SD rats （8-week age ） were randomly divided into five groups, with 10 rats in each group, which were control group （ group A ） , periodontitis group （group B）,low-dose baicalin treatment group （0.01μg/ml,group C1）,median-dose baicalin treatment group （0.1μg/ml,group C2）,high-dose baicalin treatment group （1.0μg/ml,group C3）.After 1-week treatment, these rats were sacrificed,and the specimens of gingival tissues and peripheral blood were obtained,then the expression levels of IL-8 in peripheral blood of rats were measured by cytometric bead array （ CBA） ,and the expression levels of IL-8 in gingival tissues of rats were detected by ELISA.Results The expression levels of IL-8 in peripheral blood in group B were significantly higher than those in control group （ P 〈0.05）.The expression levels of IL-8 in gingival tissues and peripheral blood in the three baicalin treatment groups were significantly higher than those in group B （ P 〈0.05） ,moreover, the expression levels of IL-8 in group C1, C2, C3 were gradually decreased in order （ P 〈0.05）.Conclusion The baicalin can decrease the expression levels of IL-8 in gingival tissues and peripheral blood of rats with periodontitis and can inhibit the destructive effects of LPS on periodontal tissues of rats.