太原市无偿献血者抗-HIV结果确认和带型分析

Analysis of the confirmation results and banding patterns of the HIV antibody of unpaid blood donors in Taiyuan City

目的分析研究太原市无偿献血者人类免疫缺陷病毒（HIV）抗体（抗-HIV）阳性标本的确认结果和带型。方法采用蛋白印迹（WB）对185例初筛阳性献血者标本进行确认试验,采用SPSS13.0分析试验结果和带型。结果确认阴性128例,不确定15例、阳性42例。确认阳性标本中,gp120、gp160、p24带型阳性率为100.00%,p31、p51和p66阳性率均大于90.00%。不确定标本中,p24阳性率最高,有12例,占80.00%;其次为gp160,有3例,占20.00%。随着S/CO值增大,带型出现率逐渐增高,最高为S/CO值〉6.00组（χ~2=35.16,P=0.009）。结论 HIV筛查试验存在假阳性。确认阳性标本多为病毒感染期。初筛阳性标本必须进行追踪确认试验,确认其是否感染。

Objective To analyze the confirmation results and banding patterns of the HIV antibody of unpaid blood donors in Taiyuan city.Methods Western blot was used to confirm the positive samples of 185 blood donors in primary screening.SPSS 13.0analysis was used to analyze the experimental results and banding patterns.Results A total of 128 negative cases were confirmed,15 cases were not confirmed and 42 cases were positive.The positive rates of banding patterns of gp120,gp160 and p24were all 100.00%,and which of p51,p66 and p31were all higher than 90.00%.In the undetermined samples,there were 12 positive cases of p24,which accounted for 80.00%,followed by gp160,3positive cases and accounted for 20.00%.With the increase of S/CO value,rates of banding patterns gradually increased,and the highest rate group was the S/CO higher than 6.00（χ2=35.16,P=0.009）.Conclusion There are some false positive results in the HIV screening test.The specimens which are confirmed positive are mostly in the virus infection period.Positive samples in primary screening must be conducted confirmed test so as to determine whether the cases are infected.