口腔鳞癌患者外周血髓源性抑制细胞和Th17细胞数量及IL-17水平增加

The number of myeloid suppressor cells,Th17 cells of peripheral blood and the serum IL-17 level increase in patients with oral squamous cell carcinoma

目的探讨口腔鳞状细胞癌(OSCC)患者外周血髓源性抑制细胞(MDSC)、白细胞介素17(IL-17)水平、Th17细胞数量及其相关性。方法募集34例OSCC患者(Ⅰ、Ⅱ期OSCC患者18例、Ⅲ、Ⅳ期OSCC患者16例)和健康自愿者16例。分别采集其空腹静脉血,流式细胞术检测外周血MDSC、Th17细胞比例,ELISA检测血清IL-17水平。结果与健康志愿者相比,OSCC患者外周血MDSC、Th17细胞比例增加,IL-17水平升高。中晚期(Ⅲ、Ⅳ期)OSCC患者MDSC、Th17细胞数量、IL-17水平均较早期(Ⅰ、Ⅱ期)OSCC患者增加。MDSC数量与IL-17水平存在相关性,而MDSC数量与Th17细胞数量无相关性。结论OSCC患者外周血MDSC、Th17细胞增加,IL-17水平升高,MDSC与IL-17可能存在相互调节作用,IL-17的主要来源并非Th17细胞,而可能来自其他固有免疫细胞。

Objective To explore the relationships in the numbers of peripheral blood myeloid derived suppressor cells（ MDSCs）,Th17 cells,and the IL-17 expression in oral squamous cell carcinoma（ OSCC） patients. Methods The fasting venous blood specimens of 34 OSCC patients and 16 heacthy donors were collected,OSCC patients includied 18 cases of stage Ⅰ and stage Ⅱ and 16 cases of stage Ⅲ and stage Ⅳ. The percentages of MDSCs and Th17 cells were determined by flow cytometry and the serum level of IL-17 was detected by ELISA. Results The percentages of MDSCs and Th17 cells,and the level of IL-17 in OSCC patients were higher than those in healthy donors. In OSCC patients,the percentages of MDSCs and Th17,and the level of IL-17 were higher in stage Ⅲ and stage Ⅳ than in stage Ⅰ and Ⅱ. The significant correlation was found between the number of MDSCs and the level of IL-17,while no correlation was found between the numbers of MDSCs and Th17 cells. Conclusion In OSCC patients,the numbers of MDSCs and Th17 cells,and the level of IL-17 increase compared with healthy donors. There is an interaction between DMSCs and Th17 cells. The IL-17 might originate from other innate immunocytes rather than Th17 cells.