磁性表面分子印迹聚合物对食品中苏丹红Ⅰ的检测

Detection of SudanⅠin Food using Magnetic Molecularly Imprinted Polymer Microspheres

本文研究了一种对苏丹红Ⅰ的检测的新方法,即磁性表面分子印迹技术。主要是以包裹二氧化硅的磁性纳米颗粒为载体,在其表面合成对苏丹红Ⅰ有特异性识别的磁性表面分子印迹聚合物。利用紫外分光光度计、红外光谱分析仪及透射电子显微镜对于所制备的苏丹红Ⅰ磁性分子印迹聚合物的磁性、形态学功能、吸附性能和特异性进行检测。实验结果表明该磁性表面分子印迹聚合物的印迹位点位于磁性载体的表层,提高与目标物质的结合能力。在外磁场条件下,磁性表面分子印迹聚合物能够轻松地达到吸附平衡并实现磁分离,降低非特异性吸附,从而提高对苏丹红Ⅰ的吸附量和选择性。最后对辣椒中含有的苏丹红Ⅰ进行了回收率检测,回收率达到80%以上。说明采用磁性表面分子印迹法对食品中苏丹红Ⅰ可快速、高效的检测。

A novel method（magnetic surface molecular imprinting technique） for the detection of Sudan I in food was investigated. A silica-wrapped magnetic nanoparticle was utilized as the carrier and on its surface the magnetic surface molecular imprinted polymer was synthesized. The magnetic properties, morphological features, adsorption properties, and specificities of the prepared Sudan I magnetic molecularly imprinted polymers were determined by ultraviolet（UV） spectrophotometry, infrared（IR） spectrometry, and transmission electron microscopy. The results showed that the imprinted sites of the magnetic surface imprinted polymer were located on the surface of the magnetic carrier, and the binding capacity with the target substance was shown to be improved. With the presence of an external magnetic field, the magnetic surface molecularly imprinted polymers easily reached adsorption equilibrium, magnetic separation was achieved, and nonspecific adsorption was reduced, thus improving the adsorption and selectivity capacity for Sudan I. Furthermore, the recovery of Sudan I in pepper was measured to be more than 80%, indicating that the magnetic surface molecular imprinting technique can be used as a novel method for the rapid and efficient detection of Sudan I in food.