摘要
目的:探讨高效液相色谱法(HPLC)测定小牛血去蛋白滴眼液中尼泊金乙酯含量的可行性。方法:利用Agilent 1100 Series高效液相色谱仪,在Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),甲醇-0.05%乙酸水溶液(65:35)作为流动相,1.0 m L·min^(-1)流速,和30℃柱温下,利用254 nm检测波长对小牛血去蛋白滴眼液中尼泊金乙酯进行定量分析。结果:尼泊金乙酯进样量在0.29~1.43μg之间呈现良好的线性关系(r=0.9999),平均加样回收率为99.80%,RSD为0.91%。结论:HPLC方法简单快速,分离效果好,可用于小牛血去蛋白滴眼液中尼泊金乙酯的定量测定。
Objective: To develop an HPLC method to determine ethylparaben in deproteinized calf blood extract eye drops. Methods: The content of ethylparaben in deproteinized calf blood extract eye drops was measured and quantified by using Agilent 1100 Series HPLC with parameters as Diamonsil C18 column (250mm×4.6mm,5μm), 30℃ column temperature, methanol-0.05% acetic acid at 1.0mL·min^-1 mobile phase, 10μL injection volume and 254 nm detection wavelength. Results: The method displayed good linearity over the concentration ranges of 0.29~1.43μg(r = 0.9999). The average recoveries of ethylparaben was 99.80%, with the RSD of 0.91%. Conclusion: The HPLC method is suitable for the determination of ethylparaben in deproteinized calf blood extract eye drops with its simple, accurate and reproducible features.
出处
《现代生物医学进展》
CAS
2016年第28期5428-5431,共4页
Progress in Modern Biomedicine
基金
湖南省科学技术厅科技计划项目(2013SK3014)