利用HPLC法测定小牛血去蛋白滴眼液中尼泊金乙酯的含量

Using HPLC Method to Quantify Ethylparaben in Deproteinized Calf Blood Extract Eye Drops

目的:探讨高效液相色谱法(HPLC)测定小牛血去蛋白滴眼液中尼泊金乙酯含量的可行性。方法:利用Agilent 1100 Series高效液相色谱仪,在Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),甲醇-0.05%乙酸水溶液(65:35)作为流动相,1.0 m L·min^(-1)流速,和30℃柱温下,利用254 nm检测波长对小牛血去蛋白滴眼液中尼泊金乙酯进行定量分析。结果:尼泊金乙酯进样量在0.29~1.43μg之间呈现良好的线性关系(r=0.9999),平均加样回收率为99.80%,RSD为0.91%。结论:HPLC方法简单快速,分离效果好,可用于小牛血去蛋白滴眼液中尼泊金乙酯的定量测定。

Objective： To develop an HPLC method to determine ethylparaben in deproteinized calf blood extract eye drops. Methods： The content of ethylparaben in deproteinized calf blood extract eye drops was measured and quantified by using Agilent 1100 Series HPLC with parameters as Diamonsil C18 column （250mm×4.6mm,5μm）, 30℃ column temperature, methanol-0.05% acetic acid at 1.0mL·min^-1 mobile phase, 10μL injection volume and 254 nm detection wavelength. Results： The method displayed good linearity over the concentration ranges of 0.29～1.43μg（r = 0.9999）. The average recoveries of ethylparaben was 99.80%, with the RSD of 0.91%. Conclusion： The HPLC method is suitable for the determination of ethylparaben in deproteinized calf blood extract eye drops with its simple, accurate and reproducible features.