化学发光法和实时荧光定量PCR法检验EB病毒的临床效果对比

Detection of EB virus by chemiluminescence method and real time quantitative PCR method

目的 分析化学发光法和实时荧光定量PCR法检测肝癌患者血清中的EB病毒载量,探讨EB病毒在肝癌检测中的意义.方法 以化学发光法和实时荧光定量PCR对正常对照组、乙肝组、丙肝组及肝癌组患者血清中的EB病毒载量进行检测,并将两种检测结果进行对比分析.结果 化学发光检测肝癌组患者EB表达水平为1.99,均显著高于乙肝（1.25,u=5.129,P＜0.05）、丙肝（1.31,u =4.497,P＜0.05）、正常组（0.03,u=10.927,P＜0.05）.实时荧光定量PCR方法检测肝癌组患者的EB表达水平为1.89,高于乙肝（1.26,u=5.295,P＜0.05）、丙肝（1.32,u=4.983,P＜0.05）及正常组（0.04,u=11.394,P＜0.05）,差异具有统计学意义;肝癌患者术前EB表达水平（化学发光：1.87;实时荧光定量PCR：1.85）明显高于术后（化学发光：0.03,u=12.873,P＜0.05）;实时荧光定量PCR：0.04,u =11.936,P＜0.05）,差异具有统计学意义.两种检测方法结果无统计学差异（P＞0.05）.结论 化学发光法和荧光定量PCR具有特异性强、灵敏度高等优点,可以用于检测肝癌的早期诊断和分期.

Objective To detect EB virus in serum of patients with hepatocellular carcinoma by chemiluminescence assay and fluorescence quantitative PCR assay and to explore the clinical meaning of EB virus measurement in hepatocellular carcinoma.Methods Chemiluminescence assay and fluorescence quantitative PCR assay were employed to measure replication level of EB virus in serum of normal healthy volunteers,patients with hepatitis B,patients with hepatitis C and patients with hepatocellular carcinoma.Results Serum EB virus replication in HCC group was significantly higher than that of the patients with hepatitis B and hepatitis C （P 〈 0.05）.Fluorescence quantitative PCR assay showed that EB virus replication in the patients of HCC was significantly higher than that in the patients with hepatitis B and hepatitis C （P.〈 0.05）.HCC patients had much higher replication level of EB virus after operation （1.87 by chemiluminescence and 1.85 by PCR） when compared to pre-operation （0.03 by chemiluminescence and 0.04 by PCR） （u =12.873,P 〈 0.05 and u =11.936,P 〈 0.05）.No significant difference of measurement results was observed between two assays （P 〉 0.05）.Conclusion Both chemiluminescence assay and fluorescence quantitative PCR assays are valuable and have strong specificity and high sensitivity in the detection of EB virus and could be applied to the early diagnosis and classification of hepatocellular carcinoma.