施氏鲟抗氧化中草药方剂的筛选

Screening of the Antioxidant Chinese Herbal Medicines for Amur Sturgeon(Acipenser schrencki Brandt)

采用口灌方法研究了22℃养殖条件下,当归、鱼腥草及一种自组中草药复方(方一)的水煎剂对1+龄施氏鲟组织中抗氧化功能相关的酶活性及活性物质含量的影响。每受试组设两个平行,每个平行30尾受试鲟,按照当归、鱼腥草及方一分别为0.3,0.54,0.8 g/kg体质量的给药剂量每日口灌给药一次,连续给药14 d,同时设立口灌蒸馏水的对照组。停药24 h后,每组取半数受试鲟高温30℃热刺激2 h后,与常温实验组共同采集血液及肝脏样品,对样品中SOD和NOS活性,MDA和NO含量进行测定。实验结果表明,所选方剂均对受试鲟血液及组织中抗氧化相关活性物质含量和酶活性有一定影响,鱼腥草和方一效果尤其突出,可有效增强受试鲟SOD活性,降低MDA含量,并对NO含量和NOS活性有一定调节作用,且在高温应激条件下仍能保持其效用,可作为鲟抗氧化中药制剂的备选药物,进行进一步的筛选和深入优化。且鱼腥草资源丰富、价格低廉、使用简单,具有良好的应用前景和优势。

In order to screen antioxidant Chinese herbal medicines for the culture of Amur sturgeon（ Acipenser schrenckii Brandt）,Angelica sinensis,Houttuynia cordata and a kind of Chinese herbal compound（ Prescription I,PI） was decocted with water and then orally given to the experimental fish of 1＋age at the temperature of 22 ℃.The activities of antioxidant related enzymes and the content of active substrates in tissues were detected after drug administration.Two parallels were set up in the experimental group,with 30 fish in each one.Angelica sinensis,Houttuynia cordata and PI were orally given to fish at a concentration of 0.3 g / kg,0.54 g / kg and 0.8 g / kg,separately once per day with a continuous administration of 14 d.The distilled water was given to fish as the control group. After the withdrawal of 24 h,half of fish in each group were thermal stimulated at30 ℃ for 2 h and then the blood and liver were sampled together with the experimental groups at 22 ℃. The activities of SOD,NOS and the contents of MDA,NO in blood and liver were determined. The results showed that 3 kinds of Chinese herbal medicines could affect the activities and contents of antioxidant related substrates in blood and liver of fish. Angelica sinensis and PI were especially useful to increase SOD activity and decrease MDA content in fish. They also had certain regulation function on NO content and NOS activity and could still keep their utilities at high temperature,which could be used as the antioxidant preparations of Chinese herbal medicines but need further screening and optimization. Besides,Angelica sinensis is rich in resources,of low price and easy to use,which therefore has a good application prospect and advantage.