基因重组型番鸭细小病毒FJM3的全基因组特征

Molecular characterization of the genome for recombinant Muscovy duck parvovirus strain FJM3

从1例未免疫雏番鸭细小病毒活疫苗与鹅细小病毒活疫苗的临床病死雏番鸭脏器中成功分离到1株番鸭细小病毒(FJM3株)。为明确其基因组特征,运用PCR方法,扩增出番鸭细小病毒FJM3株全基因组。结果表明,FJM3株基因组全长为5017nt,其基因组结构和经典番鸭细小病毒参考毒株一致。序列比对结果表明,FJM3株全基因组序列与GenBank中登录的经典MDPV参考株(FM株)核苷酸序列同源性为94.0%,与番鸭源GPV参考株(Y株)核苷酸序列同源性为85.6%。全基因组遗传进化分析表明,FJM3株处于MDPV遗传进化分支;VP1基因遗传分析表明,FJM3株处于经典MDPV遗传进化分支;VP3遗传进化表明,FJM3株处于GPV遗传进化分支。对FJM3株和参考株(FM株、Y株和SYG61v)进行遗传重组分析表明,该毒株于存在2处MDPV与GPV的基因重组现象。

In this study,Muscovy duck parvovirus strain FJM3 with natural recombination between GPVs and MDPVs was isolated from one Muscovy duck without the history of MDPV infection and GPV live attenuated vaccine inoculation. The complete genome of MDPV strain FJM3 were amplified by polymerase chain reaction methods, and then compared with typical Muscovy duck parvovirus （MDPV） and Muscovy duck origin goose parvovirus （GPV） genome sequences. The results showed that the genome length of strain FJM3 comprised 5017 nucleotides,with similar MDPV genomic features. The complete genome FJM3 was identical with MDPV isolates （strain FM） and Muscovy duck origin GPV isolates respective （strain Y） at 94. 00/40 ,and 85.60/40 ,respec- tively. Phylogenetic analysis based on complete genome and VP1 gene indicated that FJM3 shares closer relationship with typical MDPV. However, phylogenetic analysis based on VP3 gene showed that FJM3 shares closer genetic relationship with GPV isolates rather than typical MDPV isolates. Similarity plot showed that the FJM3 shared two putative natural recombination breakpoints between typical MDPVs and GPVs.