铜对大鼠肝细胞活性、自由基代谢及细胞膜电位的影响

The effects of copper on rat hepatocytes proliferation activity and its free radical metabolism and the membrane potential

通过建立大鼠肝细胞的铜胁迫模型,旨在讨论不同剂量的铜对大鼠肝细胞活性、自由基代谢及细胞膜电位的影响。以硫酸铜为试验铜源,6个不同铜质量浓度(以Cu2+计):0,50,100,200,400,800mg/L,分别加入培养液培养BRL-3A细胞24和48h后,测定不同Cu2+质量浓度、不同处理时间后细胞自由基产生、抗氧化酶活性及细胞膜电位变化。结果显示,处理质量浓度低于400 mg/L时,细胞增殖活性差异不显著(P>0.05),处理质量浓度为400,800mg/L时,细胞活性下降(P<0.05);处理质量浓度低于400 mg/L时,Cu2+质量浓度与细胞内T-SOD、GSH-Px、CP活性呈正相关,与MDA、NO含量呈负相关,质量浓度达400mg/L及以上质量浓度时,结果相反。随着Cu2+质量浓度的增加,细胞膜电位下降(P<0.05)。以上结果表明,适量的铜含量对细胞内的抗氧化酶活性具有促进作用,但高铜可能促使细胞活性下降,自由基产生增多,细胞膜电位下降。

In this research, copper poisoning model was established for further discussing different level of copper on rat hepatocyte proliferation activity, free radical metabolism and the membrane potential. The copper sulfate （CuSO4） was used as the source of copper, there were six levels of copper （Cu2＋ ） ： 0,50,100,200, 400,800 mg/L. In all of the groups, rat hepatocytes were cultured for 24 hours and 48 hours respectively, and the free radical produce,antioxidant enzyme activity and cell membrane potential were detected. The results showed that 400 and 800 mg/L copper decreased below 400 mg/L copper, the copper concentration obviously the cell proliferation activity（P〈0. 05）. When and T-SOD,GSH-Px,CP activity were positively correla- ted,and that was negatively correlated with the content of MDA and NO. The results were reverse when the concentration of copper were 400 mg/L, with the increase of copper concentration,the membrane potential decreased（P〈0. 05）. The above results showed that the proper concentration copper could promote the antioxidant enzyme activity of cells,but high copper might result in a decreasing of enzyme activity,more free radical production, and the decreasing of the hepatocyte membrane potential.