摘要
目的:建立宽叶缬草挥发油中有效成分乙酸龙脑酯的气相色谱测定法。方法:色谱条件为DB-WAX毛细管色谱柱(0.32mm×25m),程序升温60℃^10℃/min→120℃^6℃/min→140℃^10℃/min→230℃(5min),分流比为30:1,FID检测器,内标为萘。结果:乙酸龙脑酯在0.016-0.250mg范围内呈线性关系(r=0.99999),加样平均回收率为104.85%。结论:建立的方法简便、灵敏、易操作,为宽叶缬草植物资源的开发利用奠定基础。
Objecive To develop a reliable method for determination of bomyl acetate in volatile oil of Valeriana officinalis L. vat. latifolia Miq by gas chromatography. Methods A DB - WAX quartz capillary column was used and the temperature of column (0. 32mm × 25m) was programmed:程序升温60℃^10℃/min→120℃^6℃/min→140℃^10℃/min→230℃(5min), The splitting ratiowas 1:30. The FID was used as detector, and the naphthalene as internal standard. Results The standard curve was linear in the con- centration range of 0. 016 - 0. 250mg/mL. The average recovery was 104. 85%. Conclusion The new method can be used for quality assessment of leaves of Valeriana officinalis L vat. latifolia Miq. It was rapid, sensitive, precise with good reproducibility.
出处
《中国民族民间医药》
2016年第21期23-25,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
关键词
宽叶缬草
乙酸龙脑酯
气相色谱法
Valeriana officinalis L. var. latifolia Miq.
Bornyl Acetate
GC