山羊源多动物链球菌的分离与鉴定

Isolation and Identification of Streptococcus pluranimalium from Goat

从患病山羊鼻腔内分离到1株优势菌,该菌株为革兰氏阳性球菌,成对排列或为链状结构,能够发酵麦芽糖、乳糖、蔗糖和甘露醇并能产生碱性磷酸酶。对16S rDNA基因进行测序和Blast分析结果显示其与已知多动物链球菌16S rDNA序列(Y18026.1)的同源性为99%。动物回归致病性试验表明,该分离株对山羊具有较强致病性并命名为SP-1。药敏试验结果显示,该菌对氯霉素、阿米卡星、恩诺沙星、环丙沙星敏感;对阿奇霉素、红霉素、氟苯尼考、庆大霉素、阿莫西林、磺胺间甲氧嘧啶耐药。

A dominant bacterium was isolated from nasal cavity of sick goat.It was named SP-1 as this paired or chained Gram-positive coccus was highly pathogenic to goat and its 16 S rDNA has 99% sequence homology with that of known Streptococcus pluranimalium（Y18026.1）.The bacterium can ferment maltose,lactose,sucrose and mannitol and generate alkaline phosphatase.It was sensitive to chloramphenicol,amikacin,enrofloxacin and ciprofloxacin but resistant to azithromycin,erythromycin,florfenicol,gentamicin,amoxicillin and sulfamonomethoxine.