TWEAK及IL-17在强直性脊柱炎发病中的作用

Preliminary study of the roles of TWEAK and IL-17 in the pathogenesis of ankylosing spondylitis

目的通过检测肿瘤坏死因子样弱凋亡诱导因子(TWEAK)在强直性脊柱炎(AS)患者外周血单个核细胞及血浆中的表达情况并探讨其临床意义,为AS的早期诊断及治疗寻找新方法。方法采用免疫荧光方法测定AS患者与健康体检者外周血单个核细胞中TWEAK蛋白水平的表达情况,酶联免疫吸附试验(ELISA)检测AS患者及健康对照者血浆中TWEAK及IL-17水平,分析TWEAK与IL-17、CRP、ESR相关性。结果 TWEAK广泛表达于AS患者和健康对照者外周血单个核细胞,疾病活动期AS患者血浆中TWEAK水平比健康对照者明显增高,AS患者血浆IL-17水平较健康对照者明显升高,AS疾病活动期患者血浆IL-17水平较AS疾病非活动期患者明显升高。相关性分析结果显示疾病活动期AS患者血浆TWEAK水平与IL-17、CRP、ESR水平均无明显相关性,疾病活动期AS患者血浆IL-17水平与CRP、ESR水平均无明显相关性。结论 TWEAK及IL-17在AS发病中起重要作用,可能参与了AS的发病机制。

Objective To investigate the expression lever of TWEAK in blood serum and peripheral blood mononuclear cell from patients with ankylosing spondylitis （AS） and to explore the clinical significance in AS and explore new methods for the early diagnosis and treatment of AS. Methods The expressions of TWEAK protein level in peripheral blood mononuclear cells was detected by immunofluorescence. The expressions of TWEAK and IL-17 was measured by enzyme-linked immuno sorbent assay in patients with AS and healthy persons. The relationship between TWEAK level and clinical data of the patients were evaluated. Results TWEAK is widely expressed in peripheral blood mononuclear cells of AS patients and healthy controls. The serum levels of TWEAK in active stage AS patients increased significantly, compared to healthy controls. The serum levels of IL-17 in AS patients increased significantly, compared to healthy controls. The serum levels of IL-17 in active stage AS patients increased significantly, compared to non-active stage AS patients. There was no correlation between TWEAK and IL-17 ,CRP, ESR. There was no correlation between IL-17 and CRP, ESR. Conclusion The TWEAK and IL-17may play a critical role in the development of AS, and involved in the pathogenesis of AS.