2型糖尿病视网膜病变与基质金属蛋白酶-3基因多态性关系的研究

Correlation of polymorphism of MMP-3 gene with the risk of type 2 diabetic retinopathy

目的:探讨基质金属蛋白酶-3(matrix metalloproteinases-3,MMP-3)基因多态位点与承德地区人群2型糖尿病视网膜病变的遗传易感性的关系。方法:应用病例对照研究方法,选取195例糖尿病视网膜病变患者(DR组),其中非增殖性糖尿病视网膜病变(non-proliferative diabetic retinopathy,NPDR)组(n=152)和增殖性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)组(n=43),205例单纯糖尿病患者(DM组)和261例正常对照组(Control组),采用限制性片段长度多态性(restrictive fragment length polymorphism,RFLP)-聚合酶链反应(polymerase chain reaction,PCR)方法检测MMP-3的基因多态性。结果:MMP-3-1171 5A/6A的等位基因及基因型频率分布在Control组,DM组和DR组之间差异无显著性(P=0.474和P=0.407)。MMP-3-1171 5A/6A的等位基因及基因型频率分布在NPDR和PDR组之间差异无显著性(P=0.724和P=0.820)。结论:MMP-3-1171 5A/6A基因多态性与2型糖尿病视网膜病变的遗传易感性无关。

Objective： To determine the correlation between polymorphism at MMP-3 gene and risk of type 2 diabetic retinopathy （DR）. Methods： In a hospital-based case-control study, MMP-3 SNP was genotyped by polymerase chain reaction-restrictive fragment length polymorphism （PCR-RFLP） analysis in 195 diabetic retinopathy patients, 205 diabetes mellitus patients and 261 healthy persons as control. Results： There is no significant difference between the Control groups, DM group and DR group in allele or genotype distributions of MMP-3-1171 5A/6A （P=0.474 and P=0.407）. There is no significant difference between the NPDR and PDR group in allele or genotype distributions of MMP-3-1171 5A/6A （P=0.724 and P=0.820）. Conclusion： There is no significant difference between the Control groups, DM group and DRgroup in allele or genotype distributions of MMP-3 -1171 5A/6A.