槐耳清膏负性调控PI3K/Akt通路抑制裸鼠胃癌腹膜转移

Inhibition of Huaierqing Paste on gastric carcinoma with peritoneum metastasis in nude mouse based on negative regulation on PI3K/Akt pathway

目的:研究槐耳清膏抑制裸鼠胃癌腹膜转移的机制。方法:建立裸鼠胃癌模型24只,随机分为生理盐水组(n=12,每2天腹腔注射0.9%氯化钠溶液1.5m L)、槐耳清膏组(n=12,每2天腹腔注射15mg/m L的槐耳清膏1.5m L)。记录各组裸鼠体质量变化,实验性腹膜转移癌指数(e PCI),原位肿瘤相对体积(RTV),q RT-PCR检测各组胃癌组织PI3K、Akt、MMP-2、MMP-9、u PA m RNA表达情况。结果:生理盐水组与槐耳清膏组在体质量变化上无统计学差异,槐耳清膏组的实验性腹膜癌转移指数(e PCI)和RTV均显著低于生理盐水组(P<0.05),槐耳清膏组胃癌组织PI3K、Akt、MMP-2、MMP-9、u PA m RNA表达也低于生理盐水组(P<0.05)。结论:槐耳清膏可以抑制胃癌腹膜转移,可能是通过负性调控PI3K/Akt通路这一机制实现的。

Objective： To study the mechanism of Huaier inhibiting peritoneal metastasis of gastric cancer in nude mouse. Methods： 24 nude mouse models of gastric cancer were established and randomly divided into normal saline group（n=12, intraperitoneal injection of normal saline 1.5mL every 2 days） and Huaierqing Paste group（n=12, intraperitoneal injection 15mg/mL of Huaier 1.5mL every 2 days）. The indexes as body weight, experimental peritoneal carcinomatosis index（e PCI）, and relative tumor volume（RTV） were recorded. The expression of PI3 K, Akt, MMP-2, MMP-9, and u PA m RNA in tumor tissue of nude mice in each group were determined by using q RT-PCR. Results： In terms of general status, Huaierqing Paste had no obvious effect on body weight compared to normal saline group. The e PCI and RTV of Huaierqing Paste group was obviously lower than that of normal saline group（P〈0.05）, and the expression of PI3 K, Akt, MMP-2, MMP-9, and u PA m RNA in Huaierqing Paste group was also significantly lower than that of normal saline group（P〈0.05）. Conclusion： Huaierqing Paste could effectively inhibit peritoneal metastasis of gastric cancer in nude mouse, which might be related with its negative regulation on PI3K/Akt signaling pathway.