清肠愈疡汤对溃疡性结肠炎大鼠结肠黏膜ITF、NF-κB、VIP表达的影响

Effects of Qingchang Yuyang Decoction on the expression of ITF, NF-κB, VIP in colonic mucosa of ulcerative colitis of rats

目的:观察清肠愈疡汤对大鼠溃疡性结肠炎(UC)的治疗作用,并探讨其可能机制。方法:将40只鼠龄11-12周,体质量(200±20)g的SD雄性大鼠按体质量随机分为5组,分别为正常组、模型组、清肠组、5-ASA组、香连组。造模并治疗12d后,进行DAI评分,观察结肠病理改变,RT-PCR方法检测结肠组织中肠三叶因子(ITF),核转录因子-κB(NF-κB),血管活性肠肽(VIP)的表达。结果:清肠组:DAI指数在治疗后第4天1.75±0.59、第8天0.83±0.31、第12天为0.59±0.30呈逐渐降低趋势;溃疡愈合程度良好,结直肠病灶、病理形态均有明显改善,均优于其它组;清肠组中大鼠结肠组织中的ITF、VIP、NF-κB m RNA的表达量分别为0.58±0.01、0.69±0.01、3.15±0.03,与模型组、香连组、5-ASA组比较,有显著性差异(P<0.05)。结论:清肠愈疡汤可以显著改善UC大鼠症状、病理形态,通过提高大鼠结肠组织中VIP的含量,抑制NF-κB的持续激活,上调ITF在结肠组织的表达,从而达到促进溃疡愈合的效果。

Objective： To observe the effects of Qingchang Yuyang Decoction on rats of ulcerative colitis（UC） and explore its possible mechanism. Methods： Forty SD male rats aged 11-12 weeks, body weighted（200±20）g were randomly divided into 5 groups： control group, model group, Qingchang group, 5-aminosalicylic acid（5-ASA） group and Xianglian group. After 12 day＇s modeling and treatment, DAI score, pathological change of colon, expression of intestinal trefoil factor（ITF）, nuclear factor-κB（NF-κB）, and vasoactive intestinal peptide（VIP） were examined. Results： Qingchang group： DAI index showed gradual decreasing trend on the fourth day（1.75±0.59）, eighth day（0.83±0.31）, and twelfth day（0.59±0.30） after treatment; The ulcer was healing well. Colorectal lesions and pathological morphology were significantly improved. Therefore, Qingchang group was superior to other groups; it was noted that ITF, VIP, NF-κB m RNA expression in colon tissue of rats in Qingchang group were 0.58±0.01, 0.69±0.01, and 3.15±0.03, compared with the model group, Xianglian group and 5-ASA group, there was significant difference（P〈0.05）. Conclusion： Qingchang Yuyang Decoction could significantly improve the symptoms and pathological morphology in UC rats by increasing the content of VIP in the colon tissue of rats, inhibiting NF-κB activation, upregulating ITF expression in colon tissue, and eventually promote the ulcer healing.