水稻抗稻瘟病基因分子检测及抗性评价

Molecular Detection and Resistance Determination of Rice Blast-resistance Genes

为预防单一稻瘟病基因抗性容易丧失,培育持久性抗稻瘟病水稻新品种,本研究利用4份保持系、6份恢复系和3份常规稻品种为受体亲本,以75-1-127(含有稻瘟病基因Pi9)和U19(含有抗稻瘟病基因Pi25)为供体亲本,经过多代选择,获得了48份田间高代材料。叶瘟抗性鉴定结果表明,48份高代株系材料中5份表现抗,25份表现为中抗,其余均表现为中感及以上。分子标记辅助选择结果表明,19份高代株系含有抗稻瘟病基因Pi9,5份含有抗稻瘟病基因Pi25。

To avoid a possible loss of blast-resistance in rice when a single resistance gene was dependent upon for the disease prevention, new rice varieties with durable rice blast-resistance were cultivated. Forty-eight field materials of advanced-generation were obtained through a multiple-generation selection from the crosses using 4 maintainer lines, 6 restorer lines and 3 conventional rice varieties as receptor parents with 75-1-127 （harboring blast gene Pig） and U19 （harboring blast resistance gene Pi25） as donor parents. Evaluated by their resistance to the leaf blast disease, 5 lines were classified as highly resistant, 25 moderately resistant, and the remainders moderately or highly susceptible varieties. A marker-assisted selection showed that, among them, 19 advanced-generation lines carried blast-resistance gene Pig, and 5 had blast-resistance gene Pi25.