摘要
对阿氏芽胞杆菌Bacillus aryabhattai FJAT-14220发酵液中沙奎那维成分进行分析与检测。采用高效液相四级杆飞行时间质谱(liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry)分析阿氏芽胞杆菌FJAT-14220发酵液中胞外代谢物的成分。为获得代谢物的信息,首先采用MassHunter软件对原始数据进行分子特征提取,再通过Metlin数据库检索比对。在阿氏芽胞杆菌FJAT-14220发酵液中检测到883种代谢物,通过Metlin谱库搜索获得初步鉴定的有148种。其中,发现了具有生物活性的物质沙奎那维Saquinavir,其匹配得分达到93.08,占发酵液总代谢物相对含量的1.73%,保留时间为2.719 1 min,精确质量数为670.383 7。沙奎那维的发现为阿氏芽胞杆菌来源的多醚类抗生素的开发与利用提供理论依据。
Extracellular metabolites of Bacillus aryabhattai FJAT-14220 during fermentation were analyzed with liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry. Saquinavir in the fermentation broth was targeted for the study. Molecular feature extraction and database retrieval were applied to access the information on the metabolites of B. aryabhattai. A total of 883 compounds were detected with 148 of them identified. The matching score of saquinavir was 93.08 with a relative concentration of 1.73%. Its retention time was 2. 7191 min; and, accurate mass, 670. 3837. The results could be the foundation for future development and utilization of saquinavir by B. aryabhattai fermentation.
出处
《福建农业学报》
CAS
北大核心
2016年第9期981-985,共5页
Fujian Journal of Agricultural Sciences
基金
公益性行业(农业)科研专项(201303094)
福建省农业科学院杰出青年人才基金项目(2014JQ-2)
福建省科技计划项目--省属公益类科研院所基本科研专项(2015R1018-11)
关键词
液相四级杆飞行时间质谱
阿氏芽胞杆菌
胞外代谢物
沙奎那维
liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry
Bacillus aryabhattai
extracellular metabolites
saquinavir
